Inhibitors | Comment | Organism | Structure |
---|---|---|---|
sodium barbital | 3 mM, complete inhibition | Rattus norvegicus | |
sodium pentobarbital | 3 mM, complete inhibition | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Rattus norvegicus | - |
- |
nucleus | - |
Rattus norvegicus | 5634 | - |
plasma membrane | - |
Rattus norvegicus | 5886 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
enzyme extracted with 10 mM Tris, pH 8.5, 1.0% sodium deoxycholate w/v, gel filtration | Rattus norvegicus |
70000 | - |
enzyme extracted with 10 mM sodium phosphate, pH 7.0, with 1.0% sodium deoxycholate and filtered on a Sephadex column equilibrated with the neutral buffer containing 0.1% detergent, gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | the enzyme is involved in sleep | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | equally distributed in all areas of the brain | Rattus norvegicus | - |
brain | the enzyme is found in all parts of the brain in the membraneous subcellular fractions of neurons | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.85 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-30°C, not stable for more than a few days | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-AMP + H2O | 2.6% of the activity with D-glucose 6-phosphate | Rattus norvegicus | ? | - |
? | |
beta-glycerophosphate + H2O | 1.2% of the activity with D-glucose 6-phosphate | Rattus norvegicus | glycerol + phosphate | - |
? | |
D-glucose 6-phosphate + H2O | - |
Rattus norvegicus | D-glucose + phosphate | - |
? | |
diphosphate + H2O | 23% of the activity with D-glucose 6-phosphate | Rattus norvegicus | 2 phosphate | - |
? | |
additional information | the enzyme is involved in sleep | Rattus norvegicus | ? | - |
? | |
p-nitrophenylphosphate + H2O | 1.4% of the activity with D-glucose 6-phosphate | Rattus norvegicus | p-nitrophenol + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28000, SDS-PAGE | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
21 | - |
inactivated at room temperature | Rattus norvegicus |
45 | - |
10 min, 80% loss of activity | Rattus norvegicus |