Literature summary for 3.1.3.67 extracted from

Redfern, R.E.; Redfern, D.; Furgason, M.L.; Munson, M.; Ross, A.H.; Gericke, A.
PTEN phosphatase selectively binds phosphoinositides and undergoes structural changes (2008), Biochemistry, 47, 2162-2171.

Search for more literature for 3.1.3.67

Activating Compound

Activating Compound	Comment	Organism	Structure
phosphatidylinositol 4,5-biphosphate	Enhances PTEN phosphatase activity by inducing conformational change by binding to the PTEN N-terminal domain. Binding constants are in accord with enzyme kinetic measurements showing that phosphatidylinositol 4,5-bisphosphate enhances phosphatidylinositol 3,4,5-triphosphate hydrolysis more effectively than phosphatidylserine, and the binding constant of phosphatidylinositol 4,5-biphosphate of 8.14 microM is in general agreement with the phosphatidylinositol 4,5-bisphosphate concentration requires to activate the PTEN phosphatase. The magnitude of this binding constant indicates that PTEN-phosphatidylinositol 4,5-bisphosphate interactions are physiologically relevant since the effective cellular concentration of phosphatidylinositol 4,5-bisphosphate is thought to be 10 microM, and local phosphatidylinositol 4,5-bisphosphate concentrations are likely to reach even higher levels. Increasing the phosphatidylserine concentration to 25 mol results in stronger binding, but even for these higher surface charge densities, binding constant is still lower than the corresponding values for the phosphatidylcholine/phosphatidylinositol 4,5-bisphosphate vesicle system.	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
The cDNA encoding full-length 1-403 PTEN is cloned into the NdeI and XhoI sites of the pET30b vector, thereby introducing a poly-His tag at the C-terminus. Point mutations are introduced with the Quick-Change site-directed mutagenesis kit. Deletion mutant 16-403 is prepared by PCR with the Phusion DNA polymerase. PTEN proteins are expressed in Escherichia coli BL21 (DE3) cells.	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
DELTA1-15	phosphatidylinositol 4,5-biphosphate has no effect on binding of PTEN16-403	Homo sapiens
K13E	the binding of the PTEN mutant K13E, which is a tumor-derived mutation that renders PTEN inactive, is not affected by phosphatidylinositol 4,5-biphosphate	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Homo sapiens	N-terminal domain of PTEN binds anionic lipids via nonspecific electrostatic interactions	?	-	?
additional information	Homo sapiens	PTEN (phosphatase and tensin homologue deleted on chromosome 10) is a tumor suppressor that is mutated or deleted in a variety of human tumors, and even loss of only one PTEN gene profoundly affects carcinogenesis	?	-	?
additional information	Homo sapiens	PTEN binds synergistically phosphatidylinositol 4,5-biphosphate and phosphatidylserine	?	-	?
additional information	Homo sapiens	PTEN encodes a phosphatidylinositol phosphate phosphatase specific for the 3-position of the inositol ring, it influences many biological processes in both developing and adult organisms	?	-	?
phosphatidylinositol 3,4,5-triphosphate + H2O	Homo sapiens	-	phosphatidylinositol 4,5-bisphosphate + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Poly-His tag proteins are purified with a HisTrap HP kit using buffers with 10 mM mercaptoethanol, enzymes are further purified with a gel filtration column in 100 mM NaCl, 10 mM Tris, pH 7.4, and 1 mM dithiothreitol. Final purification is done with a anion exchange column in 10 mM Tris, pH 7.4 with a linear gradient from 50-600 mM NaCl.	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	N-terminal domain of PTEN binds anionic lipids via nonspecific electrostatic interactions	Homo sapiens	?	-	?
additional information	PTEN (phosphatase and tensin homologue deleted on chromosome 10) is a tumor suppressor that is mutated or deleted in a variety of human tumors, and even loss of only one PTEN gene profoundly affects carcinogenesis	Homo sapiens	?	-	?
additional information	PTEN binds synergistically phosphatidylinositol 4,5-biphosphate and phosphatidylserine	Homo sapiens	?	-	?
additional information	PTEN encodes a phosphatidylinositol phosphate phosphatase specific for the 3-position of the inositol ring, it influences many biological processes in both developing and adult organisms	Homo sapiens	?	-	?
phosphatidylinositol 3,4,5-triphosphate + H2O	-	Homo sapiens	phosphatidylinositol 4,5-bisphosphate + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
PTEN	-	Homo sapiens
PTEN phosphatase	-	Homo sapiens

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Release 2023.1 (January 2023)