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Literature summary for 3.1.3.60 extracted from

  • Duff, S.M.G.; Lefebvre, D.D.; Plaxton, W.C.
    Purification and characterization of a phosphoenolpyruvate phosphatase from Brassica nigra suspension cells (1989), Plant Physiol., 90, 734-741.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.60

Inhibitors

Inhibitors Comment Organism Structure
3-phosphoglycerate competitive; complete inhibition at 5 mM Brassica nigra
aspartate competitive Brassica nigra
CuSO4 complete inhibition at 4 mM Brassica nigra
D-fructose 1,6-bisphosphate complete inhibition at 5 mM, competitive Brassica nigra
FeCl3 complete inhibition at 4 mM Brassica nigra
glutamate competitive Brassica nigra
glyceraldhyde-3-phosphate competitive Brassica nigra
Lead acetate 70% inhibition at 8 mM Brassica nigra
MgADP 41% inhibition at 0.5 mM, competitive Brassica nigra
molybdate complete inhibition at 0.1 mM, competitive Brassica nigra
additional information several more substances found to be inhibitory, no inhibition with N-ethylmaleimide Brassica nigra
NaF uncompetitive Brassica nigra
phosphate complete inhibition at 0.5 mM, competitive Brassica nigra
ZnCl2 complete inhibition at 4 mM Brassica nigra

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 phosphoenolpyruvate 25°C, pH 5.6 Brassica nigra
0.24
-
 D-glucose 1-phosphate 25°C, pH 5.6 Brassica nigra
0.5
-
 ATP 25°C, pH 5.6 Brassica nigra
0.5
-
 2,3 diphosphoglycerate 25°C, pH 5.6 Brassica nigra
0.57
-
 4-nitrophenyl phosphate 25°C, pH 5.6 Brassica nigra
0.59
-
 D-gluconate 6-phosphate 25°C, pH 5.6 Brassica nigra
0.74
-
 ADP 25°C, pH 5.6 Brassica nigra
0.82
-
 phytic acid 25°C, pH 5.6 Brassica nigra
1.21
-
 3-phosphoglycerate 25°C, pH 5.6 Brassica nigra
2.01
-
 D-glucose 6-phosphate 25°C, pH 5.6 Brassica nigra

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1.4 fold activation at 4 mM Brassica nigra
Co2+ 1.4 fold activation at 4 mM Brassica nigra
Mg2+ 1.7 fold activation at 4 mM Brassica nigra
Mn2+ 1.4 fold activation at 4 mM Brassica nigra

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 SDS-PAGE Brassica nigra
56000
-
 gel filtration Brassica nigra

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphoenolpyruvate + H2O Brassica nigra bypasses ADP-dependent pyruvate kinase reaction during extended periods of phosphate starvation pyruvate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Brassica nigra
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Brassica nigra

Source Tissue

Source Tissue Comment Organism Textmining
leaf petiole suspension cells Brassica nigra
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 specific activity determined for all substrates Brassica nigra
380
-
 purified enzyme, 25°C, pH 5.6 Brassica nigra

Storage Stability

Storage Stability Organism
-80°C, purified enzyme, 6 months, stable Brassica nigra

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl-phosphate + H2O
-
 Brassica nigra 1-naphthol + phosphate
-
 ?
2,3-diphosphoglycerate + H2O
-
 Brassica nigra ?
-
 ?
2-phosphoglycerate + H2O poor substrate Brassica nigra glycerate + phosphate
-
 ?
3-phosphoglycerate + H2O
-
 Brassica nigra glycerate + phosphate
-
 ?
4-nitrophenyl phosphate + H2O
-
 Brassica nigra 4-nitrophenol + phosphate
-
 ?
ADP + H2O
-
 Brassica nigra ?
-
 ?
ADP-glucose + H2O poor substrate Brassica nigra ?
-
 ?
ATP + H2O
-
 Brassica nigra ?
-
 ?
D-fructose 2,6-diphosphate + H2O poor substrate Brassica nigra ?
-
 ?
D-gluconate 6-phosphate + H2O
-
 Brassica nigra D-gluconate + phosphate
-
 ?
D-glucose 1-phosphate + H2O
-
 Brassica nigra ?
-
 ?
D-glucose 6-phosphate + H2O poor substrate Brassica nigra D-glucose + phosphate
-
 ?
D-ribose 5-phosphate + H2O poor substrate Brassica nigra D-ribose + phosphate
-
 ?
O-phospho-L-threonine + H2O
-
 Brassica nigra L-threonine + phosphate
-
 ?
O-phospho-L-tyrosine + H2O
-
 Brassica nigra L-tyrosine + phosphate
-
 ?
phosphoenolpyruvate + H2O
-
 Brassica nigra pyruvate + phosphate
-
 ?
phosphoenolpyruvate + H2O bypasses ADP-dependent pyruvate kinase reaction during extended periods of phosphate starvation Brassica nigra pyruvate + phosphate
-
 ?
phytic acid + H2O
-
 Brassica nigra ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 56000, gel filtration, SDS-PAGE Brassica nigra

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 4 min, stable Brassica nigra
55
-
 4 min, 20% loss of activity Brassica nigra
60
-
 4 min, 30% loss of activity Brassica nigra

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
-
 Brassica nigra

pH Range

pH Minimum pH Maximum Comment Organism
3 7.5
-
 Brassica nigra

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.003
-
 molybdate 25°C, pH 5.6 Brassica nigra
0.05
-
 3-phosphoglycerate 25°C, pH 5.6 Brassica nigra
0.06
-
 D-fructose 1,6-bisphosphate 25°C, pH 5.6 Brassica nigra
0.085
-
 phosphate 25°C, pH 5.6 Brassica nigra