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Literature summary for 3.1.3.60 extracted from
- Purification, characterization, and subcellular localization of an acid phosphatase from black mustard cell-suspension cultures: comparison with phosphoenolpyruvate phosphatase (1991), Arch. Biochem. Biophys., 286, 226-232.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell surface | - |
Brassica nigra | 9986 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
SDS-PAGE | Brassica nigra |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + H2O | Brassica nigra | bypasses ADP-dependent pyruvate kinase reaction during extended periods of phosphate starvation | pyruvate + phosphate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brassica nigra | - |
black mustard | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Brassica nigra |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Brassica nigra |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | petiole suspension cells | Brassica nigra | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1225 | - |
purified enzyme, 25°C, pH 5.6 | Brassica nigra |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,3-diphosphoglycerate + H2O | - |
Brassica nigra | ? | - |
? | |
| 3-phosphoglycerate + H2O | - |
Brassica nigra | glycerate + phosphate | - |
? | |
| 4-nitrophenyl phosphate + H2O | - |
Brassica nigra | 4-nitrophenol + phosphate | - |
? | |
| ADP + H2O | - |
Brassica nigra | ? | - |
? | |
| ATP + H2O | - |
Brassica nigra | ? | - |
? | |
| D-gluconate 6-phosphate + H2O | - |
Brassica nigra | D-gluconate + phosphate | - |
? | |
| diphosphate + H2O | - |
Brassica nigra | 2 phosphate | - |
? | |
| O-phospho-L-tyrosine + H2O | - |
Brassica nigra | L-tyrosine + phosphate | - |
? | |
| phosphoenolpyruvate + H2O | - |
Brassica nigra | pyruvate + phosphate | - |
? | |
| phosphoenolpyruvate + H2O | bypasses ADP-dependent pyruvate kinase reaction during extended periods of phosphate starvation | Brassica nigra | pyruvate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 60000, gel filtration, SDS-PAGE | Brassica nigra |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acid phosphatase | distinct from PEP phosphatase, high activity towards phosphoenolpyruvate, in contrast to PEP phosphatase able to hydrolyze diphosphate | Brassica nigra |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.6 | - |
- |
Brassica nigra |
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Release 2023.1 (January 2023)
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