Protein Variants | Comment | Organism |
---|---|---|
D884A | mutant is not cleaved by caspase-3 in apoptotic cells | Mus musculus |
additional information | expression of the caspase-3 cleaved form of MYPT1 that lacks the C-terminal end in HeLa cells causes the dissociation of MYPT1 from actin stress fibers | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O14974 | - |
- |
Mus musculus | Q9DBR7 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | MYPT1 is cleaved by caspase-3 at Asp-884 in apoptotic cells, and the cleaved MYPT1 is strongly phosphorylated at Thr-696 and Thr-853, phosphorylation of which is known to inhibit myosin II binding | Homo sapiens |
phosphoprotein | MYPT1 is cleaved by caspase-3 at Asp-884 in apoptotic cells, and the cleaved MYPT1 is strongly phosphorylated at Thr-696 and Thr-853, phosphorylation of which is known to inhibit myosin II binding | Mus musculus |
proteolytic modification | in apoptotic cells, the myosin-binding domain of myosin phosphatase targeting subunit 1 (MYPT1) is cleaved by caspase-3 at Asp-884 | Homo sapiens |
proteolytic modification | in apoptotic cells, the myosin-binding domain of myosin phosphatase targeting subunit 1 (MYPT1) is cleaved by caspase-3 at Asp-884 | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | - |
Homo sapiens | - |
HeLa cell | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
dephosphorylation activity of myosin phosphatase immunoprecipitated from the apoptotic cells is lower than that from nonapoptotic control cells | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphorylated myosin + H2O | - |
Homo sapiens | myosin + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
myosin phosphatase targeting subunit 1 | - |
Homo sapiens |
myosin phosphatase targeting subunit 1 | - |
Mus musculus |
MYPT1 | - |
Homo sapiens |
MYPT1 | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | knockdown of MYPT1 does not cause apoptosis in normal HeLa cells but the percentage of TNF/CHX-induced apoptotic cells is increased in MYPT1-depleted cells | Homo sapiens |