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Literature summary for 3.1.3.53 extracted from

  • Iwasaki, T.; Katayama, T.; Kohama, K.; Endo, Y.; Sawasaki, T.
    Myosin phosphatase is inactivated by caspase-3 cleavage and phosphorylation of myosin phosphatase targeting subunit 1 during apoptosis (2013), Mol. Biol. Cell, 24, 748-756.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
D884A mutant is not cleaved by caspase-3 in apoptotic cells Mus musculus
additional information expression of the caspase-3 cleaved form of MYPT1 that lacks the C-terminal end in HeLa cells causes the dissociation of MYPT1 from actin stress fibers Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens O14974
-
-
Mus musculus Q9DBR7
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein MYPT1 is cleaved by caspase-3 at Asp-884 in apoptotic cells, and the cleaved MYPT1 is strongly phosphorylated at Thr-696 and Thr-853, phosphorylation of which is known to inhibit myosin II binding Homo sapiens
phosphoprotein MYPT1 is cleaved by caspase-3 at Asp-884 in apoptotic cells, and the cleaved MYPT1 is strongly phosphorylated at Thr-696 and Thr-853, phosphorylation of which is known to inhibit myosin II binding Mus musculus
proteolytic modification in apoptotic cells, the myosin-binding domain of myosin phosphatase targeting subunit 1 (MYPT1) is cleaved by caspase-3 at Asp-884 Homo sapiens
proteolytic modification in apoptotic cells, the myosin-binding domain of myosin phosphatase targeting subunit 1 (MYPT1) is cleaved by caspase-3 at Asp-884 Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
Homo sapiens
-
HeLa cell
-
Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
dephosphorylation activity of myosin phosphatase immunoprecipitated from the apoptotic cells is lower than that from nonapoptotic control cells Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphorylated myosin + H2O
-
Homo sapiens myosin + phosphate
-
?

Synonyms

Synonyms Comment Organism
myosin phosphatase targeting subunit 1
-
Homo sapiens
myosin phosphatase targeting subunit 1
-
Mus musculus
MYPT1
-
Homo sapiens
MYPT1
-
Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Homo sapiens

General Information

General Information Comment Organism
malfunction knockdown of MYPT1 does not cause apoptosis in normal HeLa cells but the percentage of TNF/CHX-induced apoptotic cells is increased in MYPT1-depleted cells Homo sapiens