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Literature summary for 3.1.3.53 extracted from

  • Kim, K.M.; Csortos, C.; Czikora, I.; Fulton, D.; Umapathy, N.S.; Olah, G.; Verin, A.D.
    Molecular characterization of myosin phosphatase in endothelium (2012), J. Cell. Physiol., 227, 1701-1708.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.53

Activating Compound

Activating Compound Comment Organism Structure
additional information the activity of the catalytic subunit of MLCP, CSbeta, towards myosin depends on its associated regulatory subunit, namely myosin PPase targeting subunit 1, MYPT1 Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
co-expression of each of two myc-tagged myosin PPase targeting subunit 1 (MYPT1) isozymes with HA-tagged MLC phosphatase catalytic subunit CS1beta in COS-7 cells, and co-expression with ERM proteins ezrin, radixin, and moesin in COS-7 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
N124A catalytic subunit mutant, catalytically inactive Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information inhibition of MLC phosphatase by either the catalytic subunit of MLC phosphatase, CS1beta, or myosin PPase targeting subunit 1, MYPT1, siRNA-based depletion results in significant attenuation of purine nucleotide (ATP and adenosine)-induced EC barrier enhancement Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
radixin + H2O Homo sapiens an ERM protein ?
-
 ?
[myosin light-chain] phosphate + H2O Homo sapiens
-
 [myosin light-chain] + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
endothelium
-
 Homo sapiens
-
pulmonary artery endothelial cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
radixin + H2O
-
 Homo sapiens ?
-
 ?
radixin + H2O an ERM protein Homo sapiens ?
-
 ?
[myosin light-chain] phosphate + H2O
-
 Homo sapiens [myosin light-chain] + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
mLC phosphatase
-
 Homo sapiens
MLCP
-
 Homo sapiens

General Information

General Information Comment Organism
additional information regulation of the enzyme involving ERM proteins ezrin, radixin, moesin, and isozymes of myosin PPase targeting subunit 1, MYPT1, overview Homo sapiens
physiological function MLCP is directly and critically involved in endothelial cell barrier regulation, enhancement, and protection, with involvement of catalytic enzyme subunit CS1beta in the endothelial cell barrier enhancement induced by extracellular purines Homo sapiens