Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.53 extracted from

  • Khromov, A.; Choudhury, N.; Stevenson, A.S.; Somlyo, A.V.; Eto, M.
    Phosphorylation-dependent autoinhibition of myosin light chain phosphatase accounts for Ca2+ sensitization force of smooth muscle contraction (2009), J. Biol. Chem., 284, 21569-21579.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.53

Cloned(Commentary)

Cloned (Comment) Organism
various parts of myosin phosphatase targeting subunit GST-fusions expressed in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
T696D part of myosin phosphatase targeting subunit, inhibitory site Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
fragment 654-880 of human myosin phosphatase targeting subunit GST-fusion
-
 Sus scrofa
fragment 697-880 of human myosin phosphatase targeting subunit GST-fusion
-
 Sus scrofa
additional information phosphorylation of myosin phosphatase targeting subunit on Thr696 and Thr853 (inhibitory sites) results in inhibition Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
GSH affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
aorta
-
 Sus scrofa
-
HeLa cell
-
 Homo sapiens
-
smooth muscle
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[myosin regulatory light chain] phosphate + H2O from chicken gizzard Sus scrofa [myosin regulatory light chain] + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
myosin light chain phosphatase
-
 Homo sapiens
myosin light chain phosphatase
-
 Sus scrofa

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0000011
-
-
 Sus scrofa phosphorylated fragment 654-880 of human myosin phosphatase targeting subunit GST-fusion
0.00006
-
-
 Sus scrofa phosphorylated fragment 697-880 of human myosin phosphatase targeting subunit GST-fusion
0.0014
-
-
 Sus scrofa unphosphorylated fragment 654-880 of human myosin phosphatase targeting subunit GST-fusion
0.0061
-
-
 Sus scrofa unphosphorylated fragment 697-880 of human myosin phosphatase targeting subunit GST-fusion