Literature summary for 3.1.3.53 extracted from

Arimura, T.; Suematsu, N.; Zhou, Y.B.; Nishimura, J.; Satoh, S.; Takeshita, A.; Kanaide, H.; Kimura, A.
Identification, characterization, and functional analysis of heart-specific myosin light chain phosphatase small subunit (2001), J. Biol. Chem., 276, 6073-6082.

Cloned(Commentary)

Cloned (Comment)	Organism
cDNAs encoding the heart-specific small regulatory subunit isoforms hHS-M21 A and B of MLCP are cloned, sequenced and expressed in Escherichia coli, the small subunit is the product of the same gene as the large subunit: MYPT2, genomic structure of the gene for hHS-M21, the genes MYPT1 and MYPT2 encoding the large myosin-binding subunit MBS are cloned and expressed in Escherichia coli, genomic organisation of the MYPT2 gene, located on chromosome 1q32	Homo sapiens

Cloned (Comment)

Organism

cDNAs encoding the heart-specific small regulatory subunit isoforms hHS-M21 A and B of MLCP are cloned, sequenced and expressed in Escherichia coli, the small subunit is the product of the same gene as the large subunit: MYPT2, genomic structure of the gene for hHS-M21, the genes MYPT1 and MYPT2 encoding the large myosin-binding subunit MBS are cloned and expressed in Escherichia coli, genomic organisation of the MYPT2 gene, located on chromosome 1q32

Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	O60237	heart-specific small regulatory subunit isoform hHS-M21 A of MLCP	-
Homo sapiens	O60237	heart-specific small regulatory subunit isoform hHS-M21 B of MLCP	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant heart-specific small regulatory subunit isoforms hHS-M21 A and B of MLCP	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
cardiac muscle	the small regulatory subunit hHS-M21 is expressed only in cardiac muscle, not in smooth muscle	Homo sapiens	-
additional information	expression profiles of the heart-specific small subunit hHS-M21 and of the large myosin-binding subunit of MLCP	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	role of the heart-specific small regulatory subunit hHS-M21 of MLCP	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
More	the heart-specific small regulatory subunit hHS-M21 of MLCP increases the Ca2+ sensitivity in muscle and binds to the large myosin-binding subunit, it plays a regulatory role in cardiac muscle contraction by its binding to the large subunit MBS, MBS encoded by the MYPT1 gene, not by MYPT2, is the main target subunit of hHS-M21	Homo sapiens