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Literature summary for 3.1.3.53 extracted from

  • Ito, M.; Feng, J.; Tsujino, S.; Inagaki, N.; Inagaki, M.; Tanaka, J.; Ichikawa, K.; Hartshorne, D.J.; Nakano, T.
    Interaction of smooth muscle myosin phosphatase with phospholipids (1997), Biochemistry, 36, 7607-7614.
    View publication on PubMed
Search for more literature for 3.1.3.53

Cloned(Commentary)

Cloned (Comment) Organism
cDNAs encoding the 133 kDa myosin-binding subunit and the 20 kDa regulatory subunit are cloned and expressed in Escherichia coli BL21(DE3) Gallus gallus

Protein Variants

Protein Variants Comment Organism
additional information mutants of the 133 kDa myosin-binding subunit Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
acidic phospholipid interaction of enzyme with acidic phospholipids inhibits activity toward phosphorylated myosin Gallus gallus
okadaic acid potent inhibitor of PP-1 and PP-2A Gallus gallus
phosphatidic acid most effective inhibitor among acidic phospholipids, followed by phosphatidylserine and phosphatidylinositol, phosphorylated myosin as substrate Gallus gallus
phosphatidylinositol phosphatidic acid is the most effective inhibitor among acidic phospholipids, followed by phosphatidylserine and phosphatidylinositol, phosphorylated myosin as substrate Gallus gallus
phosphatidylserine phosphatidic acid is the most effective inhibitor among acidic phospholipids, followed by phosphatidylserine and phosphatidylinositol, phosphorylated myosin as substrate Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol 133 kDa myosin-binding subunit Rattus norvegicus 5829
-
membrane enzyme may interact with membranes, phosphorylation by protein kinase A may modify interaction, distribution of the 130/133 kDa myosin-binding subunit at the membrane Gallus gallus 16020
-
plasma membrane 133 kDa myosin-binding subunit Rattus norvegicus 5886
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
 x * 130000 + x * 38000 + x * 20000, 130 kDa regulatory myosin-binding subunit, 38 kDa catalytic subunit, 20 kDa regulatory subunit, SDS-PAGE Gallus gallus
38000
-
 x * 130000 + x * 38000 + x * 20000, 130 kDa regulatory myosin-binding subunit, 38 kDa catalytic subunit, 20 kDa regulatory subunit, SDS-PAGE Gallus gallus
130000
-
 x * 130000 + x * 38000 + x * 20000, 130 kDa regulatory myosin-binding subunit, 38 kDa catalytic subunit, 20 kDa regulatory subunit, SDS-PAGE Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein
-
 Rattus norvegicus
phosphoprotein 130/133 kDa myosin-binding subunits M130/M133 and 20 kDa regulatory subunit M20 are phosphorylated by protein kinase A to 3 and 1 mol phosphate/mol of subunit, phosphorylation of holoenzyme decreases phospholipid binding, M130/133: C-terminal phosphorylation site involved in regulation of phospholipid binding, no phosphorylation of the catalytic subunit PP-1cdelta, protein kinase C also phosphorylates M130/M133 and M20, but no effect on binding of phospholipids Gallus gallus

Purification (Commentary)

Purification (Comment) Organism
native holoenzyme Gallus gallus
recombinant 133 kDa myosin-binding subunit and 20 kDa regulatory subunit, expressed in Escherichia coli Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
gizzard
-
 Gallus gallus
-
REF-52 cell rat embryonic fibroblast cells, mitotic, 133 kDa myosin-binding subunit Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information
-
 Rattus norvegicus ?
-
 ?
additional information enzyme binds to vesicles of acidic phospholipids, i.e. phosphatidylserine, phosphatidylinositol and phosphatidic acid, but not to neutral phospholipids, phosphatidylserine binding decreases by increasing ionic strength and Mg2+ concentration, phospholipid binding is associated with the C-terminal part of the 130/133 kDa myosin-binding subunit and the 20 kDa regulatory subunit Gallus gallus ?
-
 ?
myosin light-chain phosphate + H2O
-
 Gallus gallus myosin light-chain + phosphate
-
 ?
phosphorylated myosin + H2O
-
 Gallus gallus myosin + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 130000 + x * 38000 + x * 20000, 130 kDa regulatory myosin-binding subunit, 38 kDa catalytic subunit, 20 kDa regulatory subunit, SDS-PAGE Gallus gallus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Gallus gallus