Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.53 extracted from

  • Zhang, Y.; Mabuchi, K.; Tao, T.
    Expression in insect cells and characterization of the 110 kDa anchoring subunit of myosin light chain phosphatase (1997), Biochim. Biophys. Acta, 1343, 51-58.
    View publication on PubMed
Search for more literature for 3.1.3.53

Cloned(Commentary)

Cloned (Comment) Organism
cloning of the cDNAs encoding the 130 kDa regulatory myosin-binding subunit MLCPa, the 38 kDa catalytic subunit MLCPc and the 20 kDa regulatory subunit MLCPsr, the full-length 130 kDa subunit is expressed in SF9 insect cells using the baculovirus expression system and sequenced Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
 x * 130000 + x * 38000 + x * 20000, 130 kDa regulatory myosin-binding subunit, 38 kDa catalytic subunit, 20 kDa regulatory subunit, SDS-PAGE Gallus gallus
38000
-
 x * 130000 + x * 38000 + x * 20000, 130 kDa regulatory myosin-binding subunit, 38 kDa catalytic subunit, 20 kDa regulatory subunit, SDS-PAGE Gallus gallus
130000
-
 x * 130000 + x * 38000 + x * 20000, 130 kDa regulatory myosin-binding subunit, 38 kDa catalytic subunit, 20 kDa regulatory subunit, SDS-PAGE Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
 MLCP
-
Gallus gallus MLCP
-
 MLCP
-

Purification (Commentary)

Purification (Comment) Organism
recombinant 130 kDa regulatory myosin-binding subunit, expressed in SF9 cells Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
gizzard
-
 Gallus gallus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information 130 kDa subunit MLCPa: elongated structure with 3 globular domains connected by flexible strands Gallus gallus ?
-
 ?
additional information 130 kDa subunit MLCPa: elongated structure with 3 globular domains connected by flexible strands Gallus gallus MLCP ?
-
 ?
myosin light-chain phosphate + H2O
-
 Gallus gallus myosin light-chain + phosphate
-
 ?
myosin light-chain phosphate + H2O
-
 Gallus gallus MLCP myosin light-chain + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 130000 + x * 38000 + x * 20000, 130 kDa regulatory myosin-binding subunit, 38 kDa catalytic subunit, 20 kDa regulatory subunit, SDS-PAGE Gallus gallus