Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | enzyme SMP-IV is less sensitive than the other enzymes | Meleagris gallopavo | |
diphosphate | enzyme SMP-IV is less sensitive than the other enzymes | Meleagris gallopavo | |
NaF | enzyme SMP-IV is less sensitive than the other enzymes | Meleagris gallopavo |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
myosin light-chain | pH 7, 30°C, enzyme SMP-IV | Meleagris gallopavo | |
0.0059 | - |
heavy meromyosin | pH 7, 30°C, enzyme SMP-IV | Meleagris gallopavo |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required by enzyme SMP-II | Meleagris gallopavo |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
x * 60000 + x * 55000 + x * 38000, 38 kDa catalytic subunit, ratio 1:1:1, enzyme SMP-I, SDS-PAGE | Meleagris gallopavo |
40000 | - |
x * 58000 + x * 40000, enzyme SMP-IV | Meleagris gallopavo |
43000 | - |
1 * 43000, enzyme SMP-II | Meleagris gallopavo |
55000 | - |
x * 60000 + x * 55000 + x * 38000, 38 kDa catalytic subunit, ratio 1:1:1, enzyme SMP-I, SDS-PAGE | Meleagris gallopavo |
58000 | - |
x * 58000 + x * 40000, enzyme SMP-IV | Meleagris gallopavo |
60000 | - |
x * 60000 + x * 55000 + x * 38000, 38 kDa catalytic subunit, ratio 1:1:1, enzyme SMP-I, SDS-PAGE | Meleagris gallopavo |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
myosin light-chain phosphate + H2O | Meleagris gallopavo | reaction is a prerequisite for the actin activation of the myosin Mg2+-ATPase | myosin light-chain + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Meleagris gallopavo | - |
4 different protein phosphatases are active towards isolated myosin light-chains: SMP-I, SMP-II, SMP-III, SMP-IV | - |
Purification (Comment) | Organism |
---|---|
SMP-IV, 2000-6133fold | Meleagris gallopavo |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
gizzard smooth muscle | - |
Meleagris gallopavo | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.6 | - |
pH 7, 30°C, myosin light-chain, enzyme SMP-IV | Meleagris gallopavo |
1.84 | - |
pH 7, 30°C, heavy meromyosin, enzyme SMP-IV | Meleagris gallopavo |
Storage Stability | Organism |
---|---|
-20°C, enzyme SMP-IV, 20 mM KCl, 20 mM Tris-HCl, pH 7.4, 50% glycerol, 0.1 mM EGTA, 0.1 mM EDTA, 1 mM dithiothreitol, 0.1 mM phenylmethylsulfonyl fluoride, several months, stable | Meleagris gallopavo |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
myosin light-chain phosphate + H2O | SMP-I, -II, -III and -IV are active | Meleagris gallopavo | myosin light-chain + phosphate | - |
? | |
myosin light-chain phosphate + H2O | reaction is a prerequisite for the actin activation of the myosin Mg2+-ATPase | Meleagris gallopavo | myosin light-chain + phosphate | - |
? | |
phosphorylated heavy meromyosin + H2O | a chymotryptic fragment of myosin, only SMP-III and SMP-IV are active, SMP-I and SMP-II not | Meleagris gallopavo | heavy meromyosin + phosphate | - |
? | |
phosphorylated myosin + H2O | only SMP-III and SMP-IV are active, SMP-I and SMP-II not | Meleagris gallopavo | myosin + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000 + x * 55000 + x * 38000, 38 kDa catalytic subunit, ratio 1:1:1, enzyme SMP-I, SDS-PAGE | Meleagris gallopavo |
? | x * 58000 + x * 40000, enzyme SMP-IV | Meleagris gallopavo |
monomer | 1 * 43000, enzyme SMP-II | Meleagris gallopavo |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Meleagris gallopavo |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Meleagris gallopavo |