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Literature summary for 3.1.3.53 extracted from
- Purification and characterization of a smooth muscle myosin light chain kinase-phosphatase complex (1997), J. Biol. Chem., 272, 7034-7041.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | telokin facilitates the dissociation of the phosphatase or its catalytic subunit from myosin light chain kinase oligomers increasing the MLCPase activity of the multienzyme complex, but no effect on the activity of the purified MLCPase holoenzyme or its catalytic subunit | Meleagris gallopavo |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| myofibril | myofibrillar form of smooth muscle myosin light chain phosphatase forms a multienzyme complex with myosin light chain kinase | Meleagris gallopavo | 30016 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
x * 37000 + x * 67000, 37 kDa catalytic subunit, 67 kDa targeting subunit binds to the catalytic subunit, to myosin light chain kinase and Ca2+-independently to calmodulin, ratio of subunits 1:2, MLCPase of the multienzyme complex with myosin light chain kinase is named KAMPPase, SDS-PAGE | Meleagris gallopavo |
| 65000 | - |
catalytic subunit of KAMPPase, gel filtration | Meleagris gallopavo |
| 67000 | - |
x * 37000 + x * 67000, 37 kDa catalytic subunit, 67 kDa targeting subunit binds to the catalytic subunit, to myosin light chain kinase and Ca2+-independently to calmodulin, ratio of subunits 1:2, MLCPase of the multienzyme complex with myosin light chain kinase is named KAMPPase, SDS-PAGE | Meleagris gallopavo |
| 150000 | - |
kinase- and myosin-associated protein phosphatase KAMPPase, gel filtration | Meleagris gallopavo |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Meleagris gallopavo | together with myosin light chain kinase key regulatory enzyme of smooth muscle | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Meleagris gallopavo | - |
KAMPPase: kinase- and myosin-associated protein phosphatase, a myofibrillar form of smooth muscle myosin light chain phosphatase forms a multienzyme complex with myosin light chain kinase | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| KAMPPase, copurification of MLCPase and myosin light chain kinase activities, forming a multienzyme complex | Meleagris gallopavo |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| gizzard smooth muscle | myofibrillar form of MLCPase | Meleagris gallopavo | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | KAMPPase: kinase- and myosin-associated protein phosphatase, functional multienzyme complex composed of a myofibrillar form of smooth muscle myosin light chain phosphatase and myosin light chain kinase with or without calmodulin, regulation of the phosphatase activity | Meleagris gallopavo | ? | - |
? | |
| additional information | together with myosin light chain kinase key regulatory enzyme of smooth muscle | Meleagris gallopavo | ? | - |
? | |
| myosin light-chain phosphate + H2O | - |
Meleagris gallopavo | myosin light-chain + phosphate | - |
? | |
| phosphorylated myosin + H2O | - |
Meleagris gallopavo | myosin + phosphate | - |
? | |
| phosphorylated myosin-light chain kinase + H2O | autophosphorylated myosin light chain kinase is a good substrate for the kinase- and myosin-associated protein phosphatase KAMPPase | Meleagris gallopavo | myosin light-chain kinase + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37000 + x * 67000, 37 kDa catalytic subunit, 67 kDa targeting subunit binds to the catalytic subunit, to myosin light chain kinase and Ca2+-independently to calmodulin, ratio of subunits 1:2, MLCPase of the multienzyme complex with myosin light chain kinase is named KAMPPase, SDS-PAGE | Meleagris gallopavo |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Meleagris gallopavo |
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