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Literature summary for 3.1.3.5 extracted from

  • Martinez-Martinez, A.; Flores-Flores, C.; Campoy, F.J.; Munoz-Delgado, E.; Fini, C.; Vidal, C.J.
    Biochemical properties of 5'-nucleotidase from mouse skeletal muscle (1998), Biochim. Biophys. Acta, 1386, 16-28.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
Mus musculus
ATP
-
Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
5'-AMP
-
Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activation Mus musculus
Mg2+ activation Mus musculus
Mn2+
-
Mus musculus
Zn2+ 2.2 mol zinc per mol of protein Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
x * 74000, x * 68000, X * 51000, SDS-PAGE Mus musculus
68000
-
x * 74000, x * 68000, X * 51000, SDS-PAGE Mus musculus
74000
-
x * 74000, x * 68000, X * 51000, SDS-PAGE Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification hydrophobic anchor of glycosylphosphatidylinositol Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-AMP + H2O
-
Mus musculus adenosine + phosphate
-
?
5'-CMP + H2O 93% of the activity with 5'-AMP Mus musculus cytidine + phosphate
-
?
5'-dAMP + H2O i.e. 2'-deoxyadenosine 5'-phosphate Mus musculus deoxyadenosine + phosphate
-
?
5'-dAMP + H2O 54% of the activity with 5'-AMP Mus musculus deoxyadenosine + phosphate
-
?
5'-dGMP + H2O i.e. 2'-deoxyguanosine 5'-phosphate Mus musculus deoxyguanosine + phosphate
-
?
5'-dGMP + H2O 35% of the activity with 5'-AMP Mus musculus deoxyguanosine + phosphate
-
?
5'-GMP + H2O 120% of the activity with 5'-AMP Mus musculus guanosine + phosphate
-
?
5'-IMP + H2O 148% of the activity with 5'-AMP Mus musculus inosine + phosphate
-
?
5'-UMP + H2O 180% of the activity with 5'-AMP, preferred substrate Mus musculus uridine + phosphate
-
?

Subunits

Subunits Comment Organism
? x * 74000, x * 68000, X * 51000, SDS-PAGE Mus musculus