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Literature summary for 3.1.3.5 extracted from

  • Dornand, J.; Bonnafous, J.C.; Mani, J.C.
    Purification and properties of 5'-nucleotidase from lymphocyte plasma membranes (1978), Eur. J. Biochem., 87, 459-465.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
ATP
-
Sus scrofa
concanavalin A
-
Sus scrofa
EDTA
-
Sus scrofa
EGTA
-
Sus scrofa
Hg2+
-
Sus scrofa
Ni2+
-
Sus scrofa
Zn2+
-
Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.055
-
5'-AMP
-
Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane the active site is localized on the outer membrane surface Sus scrofa 5886
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
gel filtration Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification glycoprotein Sus scrofa

Purification (Commentary)

Purification (Comment) Organism
-
Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
lymphocyte mesenteric lymph nodes Sus scrofa
-

Storage Stability

Storage Stability Organism
4°C, 10 mM Tris-HCl, pH 7.5, 0.6% deoxycholate, 1 week, stable Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-AMP + H2O
-
Sus scrofa adenosine + phosphate
-
?
5'-CMP + H2O
-
Sus scrofa cytidine + phosphate
-
?
5'-GMP + H2O
-
Sus scrofa guanosine + phosphate
-
?
5'-IMP + H2O
-
Sus scrofa inosine + phosphate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Sus scrofa