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Literature summary for 3.1.3.5 extracted from

  • Onishi, H.; Kobayashi, T.; Kamekura, M.
    Purification and some properties of an extracellular halophilic 5'-nucleotidase from a moderate halophile, Micrococcus varians subsp. Halophilus (1984), FEMS Microbiol. Lett., 24, 303-306.
No PubMed abstract available

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Kocuria varians
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
KCl 2.5 M NaCl or KCl required Kocuria varians
NaCl 2.5 M NaCl or KCl required Kocuria varians

Organism

Organism UniProt Comment Textmining
Kocuria varians
-
subsp. halophilus
-

Purification (Commentary)

Purification (Comment) Organism
-
Kocuria varians

Storage Stability

Storage Stability Organism
5°C, 2.5 M (NH4)2SO4, 3 months, 50% loss of activity Kocuria varians

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-AMP + H2O
-
Kocuria varians adenosine + phosphate
-
?
5'-CMP + H2O 56.2% of the activity with 5'-AMP Kocuria varians cytidine + phosphate
-
?
5'-GMP + H2O 53.6% of the activity with 5'-AMP Kocuria varians guanosine + phosphate
-
?
5'-UMP + H2O 33.3% of the activity with 5'-AMP Kocuria varians uridine + phosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Kocuria varians

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Kocuria varians