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Literature summary for 3.1.3.5 extracted from
- Purification of bovine liver cytosolic 5'-nucleotidase. Kinetic and structural studies as compared to the membrane isoenzyme (1988), Eur. J. Biochem., 172, 93-99.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ADP | activation of cytosolic enzyme | Bos taurus |  |
| ATP | activation of cytosolic enzyme | Bos taurus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| agglutinin | cytosolic enzyme | Bos taurus | |
| concanavalin A | cytosolic enzyme | Bos taurus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.56 | - |
5'-UMP | cytosolic enzyme | Bos taurus | |
| 2.85 | - |
5'-AMP | cytosolic enzyme | Bos taurus | |
| 2.85 | - |
5'-IMP | cytosolic enzyme | Bos taurus | |
| 4.05 | - |
5'-CMP | cytosolic enzyme | Bos taurus | |
| 4.76 | - |
5'-GMP | cytosolic enzyme | Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Bos taurus | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
1 * 65000 + 1 * 57000, liver cytosol, SDS-PAGE | Bos taurus |
| 65000 | - |
1 * 65000 + 1 * 57000, liver cytosol, SDS-PAGE | Bos taurus |
| 130000 | - |
liver cytosol, gel filtration | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| liver cytosol | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5'-AMP + H2O | - |
Bos taurus | adenosine + phosphate | - |
? | |
| 5'-CMP + H2O | - |
Bos taurus | cytidine + phosphate | - |
? | |
| 5'-GMP + H2O | - |
Bos taurus | guanosine + phosphate | - |
? | |
| 5'-IMP + H2O | - |
Bos taurus | inosine + phosphate | - |
? | |
| 5'-UMP + H2O | - |
Bos taurus | uridine + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 65000 + 1 * 57000, liver cytosol, SDS-PAGE | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
cytosolic enzyme has a second optimum at pH 9.5 | Bos taurus |
| 9.5 | - |
cytosolic enzyme has a second optimum at pH 7.5 | Bos taurus |
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