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Literature summary for 3.1.3.5 extracted from

  • Reilly, T.J.; Calcutt, M.J.
    The class C acid phosphatase of Helicobacter pylori is a 5' nucleotidase (2004), Protein Expr. Purif., 33, 48-56.
    View publication on PubMed
Search for more literature for 3.1.3.5

Cloned(Commentary)

Cloned (Comment) Organism
cloned into pET21b and overexpressed in Escherichia coli Helicobacter pylori

Inhibitors

Inhibitors Comment Organism Structure
DTT 1 mM, about 45% inhibition Helicobacter pylori
EDTA restoration of activity by addition of divalent cations: Cu2+, Ni2+, Co2+ or Mg2+ Helicobacter pylori
HgCl2 1 mM, about 20% inhibition Helicobacter pylori
molybdate 1 mM, about 15% inhibition Helicobacter pylori
PMSF 1 mM, about 40% inhibition Helicobacter pylori
Tartrate 1 mM, about 50% inhibition Helicobacter pylori
vanadate 1 mM, about 15% inhibition Helicobacter pylori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.55
-
 5'-GMP pH 4.6, 37°C Helicobacter pylori
0.73
-
 5'-TMP pH 5.2, 37°C Helicobacter pylori
0.91
-
 5'-UMP pH 5.2, 37°C Helicobacter pylori
1.06
-
 5'-AMP pH 4.6, 37°C Helicobacter pylori
1.2
-
 5'-CMP pH 5.2, 37°C Helicobacter pylori

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Helicobacter pylori 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ restoration of activity after treatment with EDTA Helicobacter pylori
Cu2+ restoration of activity after treatment with EDTA Helicobacter pylori
Mg2+ restoration of activity after treatment with EDTA Helicobacter pylori
Ni2+ restoration of activity after treatment with EDTA Helicobacter pylori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24300
-
 x * 24300, SDS-PAGE Helicobacter pylori
72000
-
 gel filtration Helicobacter pylori

Organism

Organism UniProt Comment Textmining
Helicobacter pylori Q6UC93 ATCC strain 49503
-

Purification (Commentary)

Purification (Comment) Organism
-
 Helicobacter pylori

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.179
-
-
 Helicobacter pylori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl phosphate + H2O
-
 Helicobacter pylori 4-methylumbelliferol + phosphate
-
 ?
4-nitrophenyl phosphate + H2O about 55% of the activity with 4-methylumbelliferyl phosphate Helicobacter pylori 4-nitrophenol + phosphate
-
 ?
5'-AMP + H2O about 35% of the activity with 4-methylumbelliferyl phosphate Helicobacter pylori adenosine + phosphate
-
 ?
5'-CMP + H2O about 30% of the activity with 4-methylumbelliferyl phosphate Helicobacter pylori cytidine + phosphate
-
 ?
5'-GMP + H2O about 30% of the activity with 4-methylumbelliferyl phosphate Helicobacter pylori guanosine + phosphate
-
 ?
5'-TMP + H2O about 15% of the activity with 4-methylumbelliferyl phosphate Helicobacter pylori thymidine + phosphate
-
 ?
5'-UMP + H2O about 30% of the activity with 4-methylumbelliferyl phosphate Helicobacter pylori uridine + phosphate
-
 ?
additional information alcohols do not function as phosphate acceptors and suggests that the enzyme lacks transphosphorylation activity Helicobacter pylori ?
-
 ?

Subunits

Subunits Comment Organism
? x * 24300, SDS-PAGE Helicobacter pylori

Synonyms

Synonyms Comment Organism
class C acid phosphatase
-
 Helicobacter pylori
hppA gene product
-
 Helicobacter pylori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6
-
 reaction with AMP or GMP Helicobacter pylori
5.2
-
 reaction with CMP, TMP, UMP, 4-methylumbelliferylphosphate or p-nitrophenyl phosphate Helicobacter pylori