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Literature summary for 3.1.3.48 extracted from

  • Pei, D.; Neel, B.G.; Walsh, C.T.
    Overexpression, purification, and characterization of SHPTPa, a src homology 2-containing protein-tyrosine-phosphatase (1993), Proc. Natl. Acad. Sci. USA, 90, 1092-1096.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
enzyme SHPTP1 and its catalytic domain, lacking the N-terminal SH2-domains, expression in Escherichia coli Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
24 38 4-nitrophenyl phosphate value for the PTPase domain Homo sapiens
148
-
4-nitrophenyl phosphate value for the full length enzyme Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
1 * 45000, SHPTP1 catalytic domain, SDS-PAGE Homo sapiens
53000
-
SHPTP1, catalytic domain, gel filtration Homo sapiens
63000
-
1 * 63000, SHPTP1, full length enzyme, SDS-PAGE Homo sapiens
72000
-
SHPTP1, full length enzyme, gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16
-
-
Homo sapiens

Subunits

Subunits Comment Organism
monomer 1 * 63000, SHPTP1, full length enzyme, SDS-PAGE Homo sapiens
monomer 1 * 45000, SHPTP1 catalytic domain, SDS-PAGE Homo sapiens