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Literature summary for 3.1.3.48 extracted from

  • Zhang, Z.Y.; Clemens, J.C.; Schubert, H.L.; Stuckey, J.A.; Fischer, M.W.F.; Hume, D.M.; Saper, M.A.; Dixon, J.E.
    Expression, purification, and physicochemical characterization of a recombinant Yersinia protein tyrosine phosphatase (1992), J. Biol. Chem., 267, 23759-23766.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of Yop51 and Yop51*, a C235R point mutation and expression of the catalytic domain, residues 163-468 in Escherichia coli Yersinia enterocolitica

Protein Variants

Protein Variants Comment Organism
C235R indistinguishable from wild-type, the truncated enzyme YOP*DELTA162 has no altered catalytic properties Yersinia enterocolitica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.89 57.7 4-nitrophenyl phosphate value strongly dependent on ionic strength and pH Yersinia enterocolitica

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Yersinia enterocolitica 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
enzyme Yop51*Delta162, catalytic domain, gel filtration Yersinia enterocolitica
49800
-
enzymes Yop51, Yop51*, sedimentation equilibrium centrifugation Yersinia enterocolitica
51000
-
1 * 51000, enzymes Yop51 and Yop51*, SDS-PAGE Yersinia enterocolitica
74000
-
enzymes Yop51, Yop51*, gel filtration Yersinia enterocolitica

Organism

Organism UniProt Comment Textmining
Yersinia enterocolitica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Yersinia enterocolitica

Reaction

Reaction Comment Organism Reaction ID
[a protein]-tyrosine phosphate + H2O = [a protein]-tyrosine + phosphate mechanism Yersinia enterocolitica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
799
-
enzyme Yop51 Yersinia enterocolitica
806
-
enzyme Yop51*, point mutation C235R Yersinia enterocolitica
1233
-
enzyme Yop51 DELTA162, catalytic domain Yersinia enterocolitica

Storage Stability

Storage Stability Organism
4°C, 100 mM acetate, pH 5.7, 1 mM EDTA, ionic strength 0.15, several months, no loss of activity Yersinia enterocolitica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Yersinia enterocolitica 4-nitrophenol + phosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 35000, Yop51*DELTA162, catalytic domain, SDS-PAGE Yersinia enterocolitica
monomer 1 * 51000, enzymes Yop51 and Yop51*, SDS-PAGE Yersinia enterocolitica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2 1440 4-nitrophenyl phosphate value strongly dependent on ionic strength and pH Yersinia enterocolitica