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Literature summary for 3.1.3.48 extracted from

  • Grangeasse, C.; Doublet, P.; Vincent, C.; Vaganay, E.; Riberty, M.; Duclos, B.; Cozzone, A.J.
    Functional characterization of the low-molecular-mass phosphotyrosine-protein phosphatase of Acinetobacter johnsonii (1998), J. Mol. Biol., 278, 339-347.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Acinetobacter johnsonii

Protein Variants

Protein Variants Comment Organism
C10S complete loss of activity Acinetobacter johnsonii
R16K complete loss of activity Acinetobacter johnsonii

Inhibitors

Inhibitors Comment Organism Structure
H3VO4
-
Acinetobacter johnsonii
iodoacetic acid
-
Acinetobacter johnsonii
molybdate
-
Acinetobacter johnsonii
N-ethylmaleimide
-
Acinetobacter johnsonii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5
-
4-nitrophenyl phosphate
-
Acinetobacter johnsonii
30
-
phosphotyrosine
-
Acinetobacter johnsonii

Organism

Organism UniProt Comment Textmining
Acinetobacter johnsonii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Acinetobacter johnsonii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Acinetobacter johnsonii 4-nitrophenol + phosphate
-
?
gastrin + H2O phosphorylated on tyrosine Acinetobacter johnsonii ? + phosphate
-
?
hirudin + H2O hirudin from leech, phosphorylated on tyrosine Acinetobacter johnsonii ? + phosphate
-
?
phosphotyrosine + H2O
-
Acinetobacter johnsonii tyrosine + phosphate
-
?