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Literature summary for 3.1.3.48 extracted from

  • Lau, K.H.W.; Farley, J.R.; Baylink, D.J.
    Phosphotyrosyl-specific protein phosphatase activity of a bovine skeletal acid phosphatase isoenzyme. Comparison with the phosphotyrosyl protein phosphatase activity of skeletal alkaline phosphatase (1985), J. Biol. Chem., 260, 4653-4660.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
phosphotyrosine pH 7 Bos taurus
0.014
-
4-nitrophenyl phosphate pH 7 Bos taurus
0.064
-
4-nitrophenyl phosphate pH 8 Bos taurus
0.083
-
phosphotyrosine pH 8 Bos taurus
0.36
-
phosphotyrosine pH 9 Bos taurus
0.44
-
4-nitrophenyl phosphate pH 9 Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
bone
-
Bos taurus
-

Storage Stability

Storage Stability Organism
4°C, stable for at least 4 months Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Bos taurus 4-nitrophenol + phosphate
-
?
phosphotyrosine + H2O
-
Bos taurus tyrosine + phosphate
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
20% loss of activity after 30 min Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
substrate 4-nitrophenyl phosphate Bos taurus
6
-
phosphotyrosine Bos taurus
7
-
substrate phosphotyrosyl histone Bos taurus