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Literature summary for 3.1.3.48 extracted from

  • Li, Y.; Strohl, W.R.
    Cloning, purification, and properties of a phosphotyrosine protein phosphatase from Streptomyces coelicolor A3(2) (1996), J. Bacteriol., 178, 136-142.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces coelicolor

Inhibitors

Inhibitors Comment Organism Structure
dephostatin
-
Streptomyces coelicolor
H3VO4
-
Streptomyces coelicolor
iodoacetate
-
Streptomyces coelicolor
Phenylarsine oxide
-
Streptomyces coelicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00485
-
4-nitrophenyl phosphate
-
Streptomyces coelicolor
2.7
-
phosphotyrosine
-
Streptomyces coelicolor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18258
-
x * 18258, calculation from the sequence of the fusion protein Streptomyces coelicolor
21000
-
x * 21000, SDS-PAGE Streptomyces coelicolor

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor
-
A3(2), GenBank U37580
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Streptomyces coelicolor 4-nitrophenol + phosphate
-
?
phosphotyrosine + H2O
-
Streptomyces coelicolor tyrosine + phosphate
-
?
TSTEPQpYQPGENL + H2O
-
Streptomyces coelicolor TSTEPQYQPGENL + phosphate
-
?

Subunits

Subunits Comment Organism
? x * 18258, calculation from the sequence of the fusion protein Streptomyces coelicolor
? x * 21000, SDS-PAGE Streptomyces coelicolor

Synonyms

Synonyms Comment Organism
PtpA
-
Streptomyces coelicolor