Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bdellovibrio bacteriovorus |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 200 mM MgCl2, 100 mM Tris-HCl (pH 7.0), 8-10% (w/v) PEG 8000 | Bdellovibrio bacteriovorus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.574 | - |
Inositol hexakisphosphate | in 2 mM phytate in 50 mM sodium acetate pH 4.0, at 50°C | Bdellovibrio bacteriovorus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bdellovibrio bacteriovorus | Q6MNP0 | - |
- |
Bdellovibrio bacteriovorus HD100 | Q6MNP0 | - |
- |
Purification (Comment) | Organism |
---|---|
HiTrap Ni-IMAC column chromatography and Superdex 200 gel filtration | Bdellovibrio bacteriovorus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.041 | - |
using O-phospho-L-tyrosine as substrate, in 2 mM phytate in 50 mM sodium acetate pH 4.0, at 50°C | Bdellovibrio bacteriovorus |
0.073 | - |
using ADP as substrate, in 2 mM phytate in 50 mM sodium acetate pH 4.0, at 50°C | Bdellovibrio bacteriovorus |
0.093 | - |
using phenolphthalein diphosphate as substrate, in 2 mM phytate in 50 mM sodium acetate pH 4.0, at 50°C | Bdellovibrio bacteriovorus |
0.14 | - |
using 4-nitrophenyl phosphate as substrate, in 2 mM phytate in 50 mM sodium acetate pH 4.0, at 50°C | Bdellovibrio bacteriovorus |
0.2 | - |
using 1-naphthyl phosphate as substrate, in 2 mM phytate in 50 mM sodium acetate pH 4.0, at 50°C | Bdellovibrio bacteriovorus |
0.36 | - |
using D-fructose 1,6-diphosphate as substrate, in 2 mM phytate in 50 mM sodium acetate pH 4.0, at 50°C | Bdellovibrio bacteriovorus |
1.65 | - |
using ATP as substrate, in 2 mM phytate in 50 mM sodium acetate pH 4.0, at 50°C | Bdellovibrio bacteriovorus |
310.8 | - |
using inositol hexakisphosphate as substrate, in 2 mM phytate in 50 mM sodium acetate pH 4.0, at 50°C | Bdellovibrio bacteriovorus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-naphthyl phosphate + H2O | - |
Bdellovibrio bacteriovorus | ? | - |
? | |
4-nitrophenyl phosphate + H2O | - |
Bdellovibrio bacteriovorus | 4-nitrophenol + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | - |
Bdellovibrio bacteriovorus HD100 | 4-nitrophenol + phosphate | - |
? | |
ADP + H2O | - |
Bdellovibrio bacteriovorus | ? | - |
? | |
ADP + H2O | - |
Bdellovibrio bacteriovorus HD100 | ? | - |
? | |
ATP + H2O | - |
Bdellovibrio bacteriovorus | ? | - |
? | |
ATP + H2O | - |
Bdellovibrio bacteriovorus HD100 | ? | - |
? | |
D-fructose 1,6-diphosphate + H2O | - |
Bdellovibrio bacteriovorus | ? | - |
? | |
D-fructose 1,6-diphosphate + H2O | - |
Bdellovibrio bacteriovorus HD100 | ? | - |
? | |
inositol hexakisphosphate + H2O | highest activity | Bdellovibrio bacteriovorus | ? | - |
? | |
additional information | the enzyme does not display catalytic activity against some common protein tyrosine phosphatase substrates (O-nitrophenyl beta-D-galactopyranoside, O-phospho-L-threonine, D-fructose 6-phosphate, D-ribose 5-phosphate, D-glucose 6-phosphate, D-glucose 1-phosphate) but is highly specific for hydrolysis of phosphomonoester bonds of inositol hexakisphosphate | Bdellovibrio bacteriovorus | ? | - |
? | |
additional information | the enzyme does not display catalytic activity against some common protein tyrosine phosphatase substrates (O-nitrophenyl beta-D-galactopyranoside, O-phospho-L-threonine, D-fructose 6-phosphate, D-ribose 5-phosphate, D-glucose 6-phosphate, D-glucose 1-phosphate) but is highly specific for hydrolysis of phosphomonoester bonds of inositol hexakisphosphate | Bdellovibrio bacteriovorus HD100 | ? | - |
? | |
O-phospho-L-tyrosine + H2O | - |
Bdellovibrio bacteriovorus | ? | - |
? | |
phenolphthalein diphosphate + H2O | - |
Bdellovibrio bacteriovorus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Bd1204 | - |
Bdellovibrio bacteriovorus |
protein tyrosine phosphatase | - |
Bdellovibrio bacteriovorus |
PTP | - |
Bdellovibrio bacteriovorus |
PTP-like phytase | - |
Bdellovibrio bacteriovorus |
PTPLP | - |
Bdellovibrio bacteriovorus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Bdellovibrio bacteriovorus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 55 | more than 60% activity | Bdellovibrio bacteriovorus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
124 | - |
Inositol hexakisphosphate | in 2 mM phytate in 50 mM sodium acetate pH 4.0, at 50°C | Bdellovibrio bacteriovorus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Bdellovibrio bacteriovorus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 5 | more than 40% activity | Bdellovibrio bacteriovorus |