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Literature summary for 3.1.3.48 extracted from

  • Li, B.; Zhao, Y.; Liang, L.; Ren, H.; Xing, Y.; Chen, L.; Sun, M.; Wang, Y.; Han, Y.; Jia, H.; Huang, C.; Wu, Z.; Jia, W.
    Purification and characterization of ZmRIP1, a novel reductant-inhibited protein tyrosine phosphatase from maize (2012), Plant Physiol., 159, 671-681.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae and Arabidopsis thaliana Zea mays

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol complete inhibition at 1.8 mM Zea mays
additional information shows no sensitivity to H2O2 Zea mays
PAO complete inhibition at 0.0.5 mM Zea mays

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Zea mays 9507
-
nucleus he enzyme migrates from chloroplasts to nuclei in response to an oxidative stimulus Zea mays 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not influenced by Ca2+, Mg2+, and Mn2+ Zea mays

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
x * 42000, SDS-PAGE Zea mays

Organism

Organism UniProt Comment Textmining
Zea mays B4FJN0
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE column column chromatography, SP Sepharose column chromatography, Sephacryl S-200 gel filtration, and Sephacryl S-300 gel filtration Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
coleoptile
-
Zea mays
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2110
-
crude extract, at pH and °C Zea mays
580000
-
after 1611fold purification, at pH and °C Zea mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Zea mays 4-nitrophenol + phosphate
-
?
[EGF receptor]-tyrosine1018 phosphate + H2O highest activity Zea mays [EGF receptor]-tyrosine1018 + phosphate
-
?

Subunits

Subunits Comment Organism
? x * 42000, SDS-PAGE Zea mays

Synonyms

Synonyms Comment Organism
protein tyrosine phosphatase
-
Zea mays
PTPase
-
Zea mays
reductant-inhibited PTPase1
-
Zea mays
RIP1
-
Zea mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Zea mays

General Information

General Information Comment Organism
physiological function the enzyme functions in redox signaling Zea mays