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Literature summary for 3.1.3.48 extracted from

  • Vega, C.; Chou, S.; Engel, K.; Harrell, M.E.; Rajagopal, L.; Grundner, C.
    Structure and substrate recognition of the Staphylococcus aureus protein tyrosine phosphatase PtpA (2011), J. Mol. Biol., 413, 24-31.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
isozyme PtpA with a peptide bound in the active site mimicking a phosphotyrosine substrate, X-ray diffraction structure determination and analysis at 1.0 A resolution Staphylococcus aureus

Protein Variants

Protein Variants Comment Organism
D120A site-directed mutagenesis, the mutation abrogates activity of PtpA with the artificial phosphatase substrate 4-nitrophenol phosphate, confirming its essentiality for function Staphylococcus aureus
additional information genetic deletion of ptpA or ptpB does not affect in vitro growth or cell wall integrity Staphylococcus aureus

Inhibitors

Inhibitors Comment Organism Structure
additional information inhibitor design using the crystal structure of PtpA, overview Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
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two low-molecular-weight protein tyrosine phosphatases, PtpA and PtpB
-

Subunits

Subunits Comment Organism
More PtpA adopts a single domain, alpha/beta fold with a central four-stranded parallel beta-sheet providing the scaffold for the active site, analysis of the PtpA structural relations within the PTP family, and sequence comparison with the human isozymes, overview Staphylococcus aureus

Synonyms

Synonyms Comment Organism
protein tyrosine phosphatase
-
Staphylococcus aureus
PtpA
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Staphylococcus aureus
PtpB
-
Staphylococcus aureus

General Information

General Information Comment Organism
additional information Asp120 has an essentiality function as the general acid. Two adjacent tyrosine residues, Tyr122 and Tyr123, contribute to the deepening of the active-site pocket. Genetic deletion of ptpA or ptpB does not affect in vitro growth or cell wall integrity Staphylococcus aureus
physiological function PtpA is not essential for in vitro growth Staphylococcus aureus