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Literature summary for 3.1.3.48 extracted from

  • Wang, W.; Liu, L.; Song, X.; Mo, Y.; Komma, C.; Bellamy, H.D.; Zhao, Z.J.; Zhou, G.W.
    Crystal structure of human protein tyrosine phosphatase SHP-1 in the open conformation (2011), J. Cell. Biochem., 112, 2062-2071.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
enzyme SHP-1 expression in Escherichia coli strain BL21 (DE3) Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
SHP-1, in the open, active conformation, hanging-drop vapor diffusion, 4°C, mixing of 0.0025 ml of protein solution, containing 3.6 mg/ml protein in 25 mM Tris-HCl, pH 7.5, 0-1 M NaCl,2 mM 2-mercaptoethanol, 1 mM EDTA, with 0.0025 ml of reservoir solution, containing 1.8 M ammonium sulfate, 0.1 M glycine, 0.1 M Tris-HCl, pH 7.0, and additon of 0.5 ml 14 mM deoxy Big Chap detergent, X-ray diffraction crystal structure determination and analysis at 3.1 A resolution, molecular replacement Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme SHP-1 from Escherichia coli strain BL21 (DE3) by ion exchange chromatography and affinity chromataography on L-histidyldiazobenzylphosphonic acid, and dialysis Homo sapiens

Synonyms

Synonyms Comment Organism
More SHP-1 belongs to the family of non-receptor protein tyrosine phosphatases Homo sapiens
protein tyrosine phosphatase
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Homo sapiens
SHP-1
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Homo sapiens

General Information

General Information Comment Organism
additional information the crystal structures of the tail-truncated SHP-1 and SHP-2 reveal an autoinhibitory conformation, regulatory mechanism, overview. Interactions between two SH2 domains and between the N-SH2 and the catalytic domains can be responsible for the stabilization of SHP-1 in the open, active conformation Homo sapiens
physiological function SHP-1 generally acts as a negative regulator in a variety of cellular signaling pathways Homo sapiens