Cloned (Comment) | Organism |
---|---|
enzyme SHP-1 expression in Escherichia coli strain BL21 (DE3) | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
SHP-1, in the open, active conformation, hanging-drop vapor diffusion, 4°C, mixing of 0.0025 ml of protein solution, containing 3.6 mg/ml protein in 25 mM Tris-HCl, pH 7.5, 0-1 M NaCl,2 mM 2-mercaptoethanol, 1 mM EDTA, with 0.0025 ml of reservoir solution, containing 1.8 M ammonium sulfate, 0.1 M glycine, 0.1 M Tris-HCl, pH 7.0, and additon of 0.5 ml 14 mM deoxy Big Chap detergent, X-ray diffraction crystal structure determination and analysis at 3.1 A resolution, molecular replacement | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme SHP-1 from Escherichia coli strain BL21 (DE3) by ion exchange chromatography and affinity chromataography on L-histidyldiazobenzylphosphonic acid, and dialysis | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
More | SHP-1 belongs to the family of non-receptor protein tyrosine phosphatases | Homo sapiens |
protein tyrosine phosphatase | - |
Homo sapiens |
SHP-1 | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | the crystal structures of the tail-truncated SHP-1 and SHP-2 reveal an autoinhibitory conformation, regulatory mechanism, overview. Interactions between two SH2 domains and between the N-SH2 and the catalytic domains can be responsible for the stabilization of SHP-1 in the open, active conformation | Homo sapiens |
physiological function | SHP-1 generally acts as a negative regulator in a variety of cellular signaling pathways | Homo sapiens |