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Literature summary for 3.1.3.48 extracted from

  • Eum, J.H.; Bottjen, R.C.; Pruijssers, A.J.; Clark, K.D.; Strand, M.R.
    Characterization and kinetic analysis of protein tyrosine phosphatase-H2 from Microplitis demolitor bracovirus (2010), Insect Biochem. Mol. Biol., 40, 690-698.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene ptp-H2, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged wild-type and mutant PTP-H2 in Escherichia coli cells as non-fusion or thioredoxinfusion proteins Bracoviriform demolitoris

Protein Variants

Protein Variants Comment Organism
C236S site-directed mutagenesis, mutant PTP-H2C236S, the non-fusion mutant has almost no enzymatic activity, while the thioredoxin-fusion form has low levels of activity Bracoviriform demolitoris

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Bracoviriform demolitoris
heparin
-
Bracoviriform demolitoris
additional information the enzyme activity is diminished by increasing acetate buffer ionic strength, but PTP-H2 is unaffected by tetramisole and levamisole, as well as by okadaic acid, sodium fluoride and sodium citrate Bracoviriform demolitoris
vanadate
-
Bracoviriform demolitoris

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ increases the enzyme activity Bracoviriform demolitoris
additional information divalent cations Zn2+, Ca2+, Mg2+, and Mn2+ have little or no negative effect on activity Bracoviriform demolitoris

Organism

Organism UniProt Comment Textmining
Bracoviriform demolitoris
-
gene ptp-H2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant PTP-H2 from Escherichia coli cells by nickel affinity chromatography Bracoviriform demolitoris

Source Tissue

Source Tissue Comment Organism Textmining
additional information localization in host hemocytes following infection by Microplitis demolitor bracovirus Bracoviriform demolitoris
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ENDpYINASL + H2O
-
Bracoviriform demolitoris ENDYINASL + phosphate
-
?
additional information no activity with DADEpYLIPQQG, poor activity with 4-nitrophenyl phosphate Bracoviriform demolitoris ?
-
?

Synonyms

Synonyms Comment Organism
PTP-H2
-
Bracoviriform demolitoris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
26
-
-
Bracoviriform demolitoris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
Bracoviriform demolitoris

General Information

General Information Comment Organism
additional information PTP-H2 expression is restricted to hemocytes in Microplitis demolitor bracovirus -infected hosts Bracoviriform demolitoris