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Literature summary for 3.1.3.48 extracted from

  • Ren, L.; Chen, X.; Luechapanichkul, R.; Selner, N.G.; Meyer, T.M.; Wavreille, A.S.; Chan, R.; Iorio, C.; Zhou, X.; Neel, B.G.; Pei, D.
    Substrate specificity of protein tyrosine phosphatases 1B, RPTPalpha, SHP-1, and SHP-2 (2011), Biochemistry, 50, 2339-2356.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0032
-
WDEDFpYDWEF pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
0.0063
-
WDEDFpYDWEF pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
0.013
-
FDIDIpYAA pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
0.017
-
DWEDFpYAA pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
0.022
-
AWSpYADpYAA pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
0.023
-
DFEDFpYAA pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
0.027
-
EIFDFpYAA pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
0.028
-
FYDIDpYAA pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
0.028
-
DAEDFpYAA pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
0.031
-
FDEDFpYAA pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
0.034
-
ADEDFpYAA pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
0.039
-
FDIDIpYAA pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
0.043
-
WDEDFpYAA pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
0.045
-
WAGDDpYAA pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
0.046
-
WDEDFpYSASA pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
0.048
-
SASASpYDWEF pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
0.053
-
WDEDFpYSASA pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
0.054
-
EIFDFpYAA pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
0.058
-
AKFEDTpYAA pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
0.066
-
FYDIDpYAA pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
0.068
-
WAGDDpYAA pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
0.075
-
AVWEFpYpYAA pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
0.091
-
AYTEpYTpYAA pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
0.15
-
WDEDFpYRWKF pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
0.18
-
SASASpYDWEF pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
0.21
-
WDEDFpYRWKF pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
0.49
-
ARKRIpYAA pH 7.4, temperature not specified in the publication, RPTPalpha Homo sapiens
0.53
-
SASASpYSASA pH 7.4, temperature not specified in the publication, RPTPalpha Homo sapiens
0.56
-
WRKRFpYDWEF pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
0.77
-
LIEDNEpYTARQGA pH 7.4, temperature not specified in the publication, RPTPalpha Homo sapiens
0.78
-
WRKRFpYDWEF pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
1.07
-
TATEPQpYQPGEN pH 7.4, temperature not specified in the publication, RPTPalpha Homo sapiens
1.16
-
TSTEPQpYQPGENL pH 7.4, temperature not specified in the publication, RPTPalpha Homo sapiens
1.2
-
RRISTpYAA pH 7.4, temperature not specified in the publication, RPTPalpha Homo sapiens
1.4
-
EADTApYAA pH 7.4, temperature not specified in the publication, RPTPalpha Homo sapiens
1.99
-
SASASpYSASA pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
2.3
-
SASASpYSASA pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
2.6
-
AAAAApYRHRR pH 7.4, temperature not specified in the publication, RPTPalpha Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
AAAAApYEEVH + H2O substrate of RPTPalpha Homo sapiens AAAAAYEEVH + phosphate
-
?
AAAAApYRHRR + H2O substrate of RPTPalpha Homo sapiens AAAAAYRHRR + phosphate
-
?
ADEDFpYAA + H2O substrate of SHP-2 Homo sapiens ADEDFYAA + phosphate
-
?
AKFEDTpYAA + H2O substrate of SHP-1 Homo sapiens AKFEDTYAA + phosphate
-
?
ARKRIpYAA + H2O substrate of RPTPalpha Homo sapiens ARKRIYAA + phosphate
-
?
ASSDDpYAA + H2O substrate of RPTPalpha Homo sapiens ASSDDYAA + phosphate
-
?
AVWEFpYpYAA + H2O substrate of SHP-1 Homo sapiens ? + phosphate
-
?
AWSpYADpYAA + H2O substrate of SHP-1 Homo sapiens ? + phosphate
-
?
AYTEpYTpYAA + H2O substrate of SHP-1 Homo sapiens ? + phosphate
-
?
DAEDFpYAA + H2O substrate of SHP-2 Homo sapiens DAEDFYAA + phosphate
-
?
DFEDFpYAA + H2O substrate of SHP-2 Homo sapiens DFEDFYAA + phosphate
-
?
DWEDFpYAA + H2O substrate of SHP-2 Homo sapiens DWEDFYAA + phosphate
-
?
EADTApYAA + H2O substrate of RPTPalpha Homo sapiens EADTAYAA + phosphate
-
?
EIFDFpYAA + H2O substrate of SHP-1 and SHP-2 Homo sapiens EIFDFYAA + phosphate
-
?
FDEDFpYAA + H2O substrate of SHP-2 Homo sapiens FDEDFYAA + phosphate
-
?
FDIDIpYAA + H2O substrate of SHP-1 and SHP-2 Homo sapiens FDIDIYAA + phosphate
-
?
FYDIDpYAA + H2O substrate of SHP-1 and SHP-2 Homo sapiens FYDIDYAA + phosphate
-
?
LIEDNEpYTARQGA + H2O substrate of RPTPalpha Homo sapiens LIEDNEYTARQGA + phosphate
-
?
additional information the PTP1B catalytic domain has modest preference for acidic residues on both sides of phosphotyrosine, is highly active toward multiply phosphorylated peptides, but disfavors basic residues at any position, a Gly at the phosphotyrosine-1 position, or a Pro at the pY-/-1 position. By contrast, SHP-1 and SHP-2 share similar but much narrower substrate specificities, with a strong preference for acidic and aromatic hydrophobic amino acids on both sides of the phosphotyrosine residue. An efficient SHP-1/2 substrate generally contains two or more acidic residues on the N-terminal side and one or more acidic residues on the C-terminal side of pY but no basic residues, substrate specificities of the protein tyrosine phosphatases PTP1B, RPTPalpha, SHP-1, and SHP-2, and reported PTP1B, SHP-1, and SHP-2 substrates and their dephosphorylation sites, overview. The catalytic domain of RPTPalpha has very weak sequence specificity and is approximately 2 orders of magnitude less active than the other three enzyme variants Homo sapiens ?
-
?
RRISTpYAA + H2O substrate of RPTPalpha Homo sapiens RRISTYAA + phosphate
-
?
SASASpYDWEF + H2O substrate of SHP-1 and SHP-2 Homo sapiens SASASYDWEF + phosphate
-
?
SASASpYSASA + H2O substrate of RPTPalpha Homo sapiens SASASYSASA + phosphate
-
?
SASASpYSASA + H2O substrate of SHP-1 and SHP-2 Homo sapiens SASASYSASA + phosphate
-
?
TATEPQpYQPGEN + H2O substrate of RPTPalpha Homo sapiens TATEPQYQPGEN + phosphate
-
?
TATEPQpYQPGENL + H2O substrate of RPTPalpha Homo sapiens TATEPQYQPGENL + phosphate
-
?
TSTEPQpYQPGENL + H2O substrate of RPTPalpha Homo sapiens TSTEPQYQPGENL + phosphate
-
?
WAGDDpYAA + H2O substrate of SHP-1 and SHP-2 Homo sapiens WAGDDYAA + phosphate
-
?
WDEDFpYAA + H2O substrate of SHP-2 Homo sapiens WDEDFYAA + phosphate
-
?
WDEDFpYDWEF + H2O substrate of SHP-1 and SHP-2 Homo sapiens WDEDFYDWEF + phosphate
-
?
WDEDFpYRWKF + H2O substrate of SHP-1 and SHP-2 Homo sapiens WDEDFYRWKF + phosphate
-
?
WDEDFpYSASA + H2O substrate of RPTPalpha Homo sapiens WDEDFYSASA + phosphate
-
?
WDEDFpYSASA + H2O substrate of SHP-1 and SHP-2 Homo sapiens WDEDFYSASA + phosphate
-
?
WRKRFpYDWEF + H2O substrate of SHP-1 and SHP-2 Homo sapiens WRKRFYDWEF + phosphate
-
?

Synonyms

Synonyms Comment Organism
protein tyrosine phosphatase
-
Homo sapiens
PTP1B
-
Homo sapiens
RPTPalpha
-
Homo sapiens
SHP-1
-
Homo sapiens
SHP-2
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3
-
WRKRFpYDWEF pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
1.7
-
WRKRFpYDWEF pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
2 8 ADEDFpYAA pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
3 6 WAGDDpYAA pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
8.3
-
LIEDNEpYTARQGA pH 7.4, temperature not specified in the publication, RPTPalpha Homo sapiens
8.9
-
TSTEPQpYQPGENL pH 7.4, temperature not specified in the publication, RPTPalpha Homo sapiens
9.7
-
TATEPQpYQPGEN pH 7.4, temperature not specified in the publication, RPTPalpha Homo sapiens
12
-
SASASpYDWEF pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
13
-
AWSpYADpYAA pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
17
-
AYTEpYTpYAA pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
19
-
AKFEDTpYAA pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
23
-
SASASpYSASA pH 7.4, temperature not specified in the publication, RPTPalpha Homo sapiens
24
-
AVWEFpYpYAA pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
26
-
EADTApYAA pH 7.4, temperature not specified in the publication, RPTPalpha Homo sapiens
26
-
SASASpYSASA pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
26
-
EIFDFpYAA pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
30
-
AAAAApYRHRR pH 7.4, temperature not specified in the publication, RPTPalpha Homo sapiens
30
-
FDIDIpYAA pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
30
-
FDEDFpYAA pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
30
-
DWEDFpYAA pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
30
-
DAEDFpYAA pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
32
-
EIFDFpYAA pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
33
-
FDIDIpYAA pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
35
-
SASASpYDWEF pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
37
-
WDEDFpYSASA pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
37
-
DFEDFpYAA pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
39
-
ARKRIpYAA pH 7.4, temperature not specified in the publication, RPTPalpha Homo sapiens
42
-
WDEDFpYRWKF pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
42
-
SASASpYSASA pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
43
-
WDEDFpYDWEF pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
48
-
WDEDFpYAA pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
49
-
RRISTpYAA pH 7.4, temperature not specified in the publication, RPTPalpha Homo sapiens
50
-
FYDIDpYAA pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
50
-
WDEDFpYSASA pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
57
-
WAGDDpYAA pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
66
-
FYDIDpYAA pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
73
-
WDEDFpYRWKF pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
75
-
WDEDFpYDWEF pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0022
-
WRKRFpYDWEF pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
0.0023
-
WRKRFpYDWEF pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
0.013
-
SASASpYSASA pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
0.018
-
SASASpYSASA pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
0.19
-
SASASpYDWEF pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
0.25
-
SASASpYDWEF pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
0.28
-
WDEDFpYRWKF pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
0.34
-
AKFEDTpYAA pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
0.34
-
WDEDFpYRWKF pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
0.48
-
EIFDFpYAA pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
0.53
-
WAGDDpYAA pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
0.58
-
AWSpYADpYAA pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
0.7
-
WDEDFpYSASA pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
0.75
-
FYDIDpYAA pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
0.77
-
FDIDIpYAA pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
0.82
-
ADEDFpYAA pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
1
-
FDEDFpYAA pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
1.1
-
WDEDFpYSASA pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
1.1
-
WDEDFpYAA pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
1.1
-
DAEDFpYAA pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
1.2
-
EIFDFpYAA pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
1.3
-
WAGDDpYAA pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
1.6
-
DFEDFpYAA pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
1.7
-
DWEDFpYAA pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
1.9
-
AYTEpYTpYAA pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
2.4
-
FYDIDpYAA pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
2.5
-
FDIDIpYAA pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
3.3
-
AVWEFpYpYAA pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
6.8
-
WDEDFpYDWEF pH 7.4, temperature not specified in the publication, SHP-2 Homo sapiens
7.7
-
TSTEPQpYQPGENL pH 7.4, temperature not specified in the publication, RPTPalpha Homo sapiens
9.1
-
TATEPQpYQPGEN pH 7.4, temperature not specified in the publication, RPTPalpha Homo sapiens
11
-
LIEDNEpYTARQGA pH 7.4, temperature not specified in the publication, RPTPalpha Homo sapiens
12
-
AAAAApYRHRR pH 7.4, temperature not specified in the publication, RPTPalpha Homo sapiens
13
-
ASSDDpYAA pH 7.4, temperature not specified in the publication, RPTPalpha Homo sapiens
19
-
EADTApYAA pH 7.4, temperature not specified in the publication, RPTPalpha Homo sapiens
23
-
WDEDFpYDWEF pH 7.4, temperature not specified in the publication, SHP-1 Homo sapiens
39
-
AAAAApYEEVH pH 7.4, temperature not specified in the publication, RPTPalpha Homo sapiens
41
-
RRISTpYAA pH 7.4, temperature not specified in the publication, RPTPalpha Homo sapiens
43
-
SASASpYSASA pH 7.4, temperature not specified in the publication, RPTPalpha Homo sapiens
69
-
WDEDFpYSASA pH 7.4, temperature not specified in the publication, RPTPalpha Homo sapiens
81
-
ARKRIpYAA pH 7.4, temperature not specified in the publication, RPTPalpha Homo sapiens