KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0032 | - |
WDEDFpYDWEF | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
0.0063 | - |
WDEDFpYDWEF | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
0.013 | - |
FDIDIpYAA | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
0.017 | - |
DWEDFpYAA | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
0.022 | - |
AWSpYADpYAA | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
0.023 | - |
DFEDFpYAA | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
0.027 | - |
EIFDFpYAA | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
0.028 | - |
FYDIDpYAA | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
0.028 | - |
DAEDFpYAA | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
0.031 | - |
FDEDFpYAA | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
0.034 | - |
ADEDFpYAA | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
0.039 | - |
FDIDIpYAA | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
0.043 | - |
WDEDFpYAA | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
0.045 | - |
WAGDDpYAA | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
0.046 | - |
WDEDFpYSASA | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
0.048 | - |
SASASpYDWEF | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
0.053 | - |
WDEDFpYSASA | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
0.054 | - |
EIFDFpYAA | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
0.058 | - |
AKFEDTpYAA | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
0.066 | - |
FYDIDpYAA | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
0.068 | - |
WAGDDpYAA | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
0.075 | - |
AVWEFpYpYAA | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
0.091 | - |
AYTEpYTpYAA | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
0.15 | - |
WDEDFpYRWKF | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
0.18 | - |
SASASpYDWEF | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
0.21 | - |
WDEDFpYRWKF | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
0.49 | - |
ARKRIpYAA | pH 7.4, temperature not specified in the publication, RPTPalpha | Homo sapiens | |
0.53 | - |
SASASpYSASA | pH 7.4, temperature not specified in the publication, RPTPalpha | Homo sapiens | |
0.56 | - |
WRKRFpYDWEF | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
0.77 | - |
LIEDNEpYTARQGA | pH 7.4, temperature not specified in the publication, RPTPalpha | Homo sapiens | |
0.78 | - |
WRKRFpYDWEF | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
1.07 | - |
TATEPQpYQPGEN | pH 7.4, temperature not specified in the publication, RPTPalpha | Homo sapiens | |
1.16 | - |
TSTEPQpYQPGENL | pH 7.4, temperature not specified in the publication, RPTPalpha | Homo sapiens | |
1.2 | - |
RRISTpYAA | pH 7.4, temperature not specified in the publication, RPTPalpha | Homo sapiens | |
1.4 | - |
EADTApYAA | pH 7.4, temperature not specified in the publication, RPTPalpha | Homo sapiens | |
1.99 | - |
SASASpYSASA | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
2.3 | - |
SASASpYSASA | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
2.6 | - |
AAAAApYRHRR | pH 7.4, temperature not specified in the publication, RPTPalpha | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
AAAAApYEEVH + H2O | substrate of RPTPalpha | Homo sapiens | AAAAAYEEVH + phosphate | - |
? | |
AAAAApYRHRR + H2O | substrate of RPTPalpha | Homo sapiens | AAAAAYRHRR + phosphate | - |
? | |
ADEDFpYAA + H2O | substrate of SHP-2 | Homo sapiens | ADEDFYAA + phosphate | - |
? | |
AKFEDTpYAA + H2O | substrate of SHP-1 | Homo sapiens | AKFEDTYAA + phosphate | - |
? | |
ARKRIpYAA + H2O | substrate of RPTPalpha | Homo sapiens | ARKRIYAA + phosphate | - |
? | |
ASSDDpYAA + H2O | substrate of RPTPalpha | Homo sapiens | ASSDDYAA + phosphate | - |
? | |
AVWEFpYpYAA + H2O | substrate of SHP-1 | Homo sapiens | ? + phosphate | - |
? | |
AWSpYADpYAA + H2O | substrate of SHP-1 | Homo sapiens | ? + phosphate | - |
? | |
AYTEpYTpYAA + H2O | substrate of SHP-1 | Homo sapiens | ? + phosphate | - |
? | |
DAEDFpYAA + H2O | substrate of SHP-2 | Homo sapiens | DAEDFYAA + phosphate | - |
? | |
DFEDFpYAA + H2O | substrate of SHP-2 | Homo sapiens | DFEDFYAA + phosphate | - |
? | |
DWEDFpYAA + H2O | substrate of SHP-2 | Homo sapiens | DWEDFYAA + phosphate | - |
? | |
EADTApYAA + H2O | substrate of RPTPalpha | Homo sapiens | EADTAYAA + phosphate | - |
? | |
EIFDFpYAA + H2O | substrate of SHP-1 and SHP-2 | Homo sapiens | EIFDFYAA + phosphate | - |
? | |
FDEDFpYAA + H2O | substrate of SHP-2 | Homo sapiens | FDEDFYAA + phosphate | - |
? | |
FDIDIpYAA + H2O | substrate of SHP-1 and SHP-2 | Homo sapiens | FDIDIYAA + phosphate | - |
? | |
FYDIDpYAA + H2O | substrate of SHP-1 and SHP-2 | Homo sapiens | FYDIDYAA + phosphate | - |
? | |
LIEDNEpYTARQGA + H2O | substrate of RPTPalpha | Homo sapiens | LIEDNEYTARQGA + phosphate | - |
? | |
additional information | the PTP1B catalytic domain has modest preference for acidic residues on both sides of phosphotyrosine, is highly active toward multiply phosphorylated peptides, but disfavors basic residues at any position, a Gly at the phosphotyrosine-1 position, or a Pro at the pY-/-1 position. By contrast, SHP-1 and SHP-2 share similar but much narrower substrate specificities, with a strong preference for acidic and aromatic hydrophobic amino acids on both sides of the phosphotyrosine residue. An efficient SHP-1/2 substrate generally contains two or more acidic residues on the N-terminal side and one or more acidic residues on the C-terminal side of pY but no basic residues, substrate specificities of the protein tyrosine phosphatases PTP1B, RPTPalpha, SHP-1, and SHP-2, and reported PTP1B, SHP-1, and SHP-2 substrates and their dephosphorylation sites, overview. The catalytic domain of RPTPalpha has very weak sequence specificity and is approximately 2 orders of magnitude less active than the other three enzyme variants | Homo sapiens | ? | - |
? | |
RRISTpYAA + H2O | substrate of RPTPalpha | Homo sapiens | RRISTYAA + phosphate | - |
? | |
SASASpYDWEF + H2O | substrate of SHP-1 and SHP-2 | Homo sapiens | SASASYDWEF + phosphate | - |
? | |
SASASpYSASA + H2O | substrate of RPTPalpha | Homo sapiens | SASASYSASA + phosphate | - |
? | |
SASASpYSASA + H2O | substrate of SHP-1 and SHP-2 | Homo sapiens | SASASYSASA + phosphate | - |
? | |
TATEPQpYQPGEN + H2O | substrate of RPTPalpha | Homo sapiens | TATEPQYQPGEN + phosphate | - |
? | |
TATEPQpYQPGENL + H2O | substrate of RPTPalpha | Homo sapiens | TATEPQYQPGENL + phosphate | - |
? | |
TSTEPQpYQPGENL + H2O | substrate of RPTPalpha | Homo sapiens | TSTEPQYQPGENL + phosphate | - |
? | |
WAGDDpYAA + H2O | substrate of SHP-1 and SHP-2 | Homo sapiens | WAGDDYAA + phosphate | - |
? | |
WDEDFpYAA + H2O | substrate of SHP-2 | Homo sapiens | WDEDFYAA + phosphate | - |
? | |
WDEDFpYDWEF + H2O | substrate of SHP-1 and SHP-2 | Homo sapiens | WDEDFYDWEF + phosphate | - |
? | |
WDEDFpYRWKF + H2O | substrate of SHP-1 and SHP-2 | Homo sapiens | WDEDFYRWKF + phosphate | - |
? | |
WDEDFpYSASA + H2O | substrate of RPTPalpha | Homo sapiens | WDEDFYSASA + phosphate | - |
? | |
WDEDFpYSASA + H2O | substrate of SHP-1 and SHP-2 | Homo sapiens | WDEDFYSASA + phosphate | - |
? | |
WRKRFpYDWEF + H2O | substrate of SHP-1 and SHP-2 | Homo sapiens | WRKRFYDWEF + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
protein tyrosine phosphatase | - |
Homo sapiens |
PTP1B | - |
Homo sapiens |
RPTPalpha | - |
Homo sapiens |
SHP-1 | - |
Homo sapiens |
SHP-2 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
WRKRFpYDWEF | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
1.7 | - |
WRKRFpYDWEF | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
2 | 8 | ADEDFpYAA | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
3 | 6 | WAGDDpYAA | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
8.3 | - |
LIEDNEpYTARQGA | pH 7.4, temperature not specified in the publication, RPTPalpha | Homo sapiens | |
8.9 | - |
TSTEPQpYQPGENL | pH 7.4, temperature not specified in the publication, RPTPalpha | Homo sapiens | |
9.7 | - |
TATEPQpYQPGEN | pH 7.4, temperature not specified in the publication, RPTPalpha | Homo sapiens | |
12 | - |
SASASpYDWEF | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
13 | - |
AWSpYADpYAA | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
17 | - |
AYTEpYTpYAA | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
19 | - |
AKFEDTpYAA | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
23 | - |
SASASpYSASA | pH 7.4, temperature not specified in the publication, RPTPalpha | Homo sapiens | |
24 | - |
AVWEFpYpYAA | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
26 | - |
EADTApYAA | pH 7.4, temperature not specified in the publication, RPTPalpha | Homo sapiens | |
26 | - |
SASASpYSASA | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
26 | - |
EIFDFpYAA | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
30 | - |
AAAAApYRHRR | pH 7.4, temperature not specified in the publication, RPTPalpha | Homo sapiens | |
30 | - |
FDIDIpYAA | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
30 | - |
FDEDFpYAA | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
30 | - |
DWEDFpYAA | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
30 | - |
DAEDFpYAA | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
32 | - |
EIFDFpYAA | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
33 | - |
FDIDIpYAA | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
35 | - |
SASASpYDWEF | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
37 | - |
WDEDFpYSASA | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
37 | - |
DFEDFpYAA | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
39 | - |
ARKRIpYAA | pH 7.4, temperature not specified in the publication, RPTPalpha | Homo sapiens | |
42 | - |
WDEDFpYRWKF | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
42 | - |
SASASpYSASA | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
43 | - |
WDEDFpYDWEF | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
48 | - |
WDEDFpYAA | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
49 | - |
RRISTpYAA | pH 7.4, temperature not specified in the publication, RPTPalpha | Homo sapiens | |
50 | - |
FYDIDpYAA | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
50 | - |
WDEDFpYSASA | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
57 | - |
WAGDDpYAA | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
66 | - |
FYDIDpYAA | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
73 | - |
WDEDFpYRWKF | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
75 | - |
WDEDFpYDWEF | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0022 | - |
WRKRFpYDWEF | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
0.0023 | - |
WRKRFpYDWEF | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
0.013 | - |
SASASpYSASA | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
0.018 | - |
SASASpYSASA | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
0.19 | - |
SASASpYDWEF | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
0.25 | - |
SASASpYDWEF | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
0.28 | - |
WDEDFpYRWKF | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
0.34 | - |
AKFEDTpYAA | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
0.34 | - |
WDEDFpYRWKF | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
0.48 | - |
EIFDFpYAA | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
0.53 | - |
WAGDDpYAA | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
0.58 | - |
AWSpYADpYAA | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
0.7 | - |
WDEDFpYSASA | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
0.75 | - |
FYDIDpYAA | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
0.77 | - |
FDIDIpYAA | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
0.82 | - |
ADEDFpYAA | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
1 | - |
FDEDFpYAA | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
1.1 | - |
WDEDFpYSASA | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
1.1 | - |
WDEDFpYAA | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
1.1 | - |
DAEDFpYAA | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
1.2 | - |
EIFDFpYAA | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
1.3 | - |
WAGDDpYAA | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
1.6 | - |
DFEDFpYAA | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
1.7 | - |
DWEDFpYAA | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
1.9 | - |
AYTEpYTpYAA | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
2.4 | - |
FYDIDpYAA | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
2.5 | - |
FDIDIpYAA | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
3.3 | - |
AVWEFpYpYAA | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
6.8 | - |
WDEDFpYDWEF | pH 7.4, temperature not specified in the publication, SHP-2 | Homo sapiens | |
7.7 | - |
TSTEPQpYQPGENL | pH 7.4, temperature not specified in the publication, RPTPalpha | Homo sapiens | |
9.1 | - |
TATEPQpYQPGEN | pH 7.4, temperature not specified in the publication, RPTPalpha | Homo sapiens | |
11 | - |
LIEDNEpYTARQGA | pH 7.4, temperature not specified in the publication, RPTPalpha | Homo sapiens | |
12 | - |
AAAAApYRHRR | pH 7.4, temperature not specified in the publication, RPTPalpha | Homo sapiens | |
13 | - |
ASSDDpYAA | pH 7.4, temperature not specified in the publication, RPTPalpha | Homo sapiens | |
19 | - |
EADTApYAA | pH 7.4, temperature not specified in the publication, RPTPalpha | Homo sapiens | |
23 | - |
WDEDFpYDWEF | pH 7.4, temperature not specified in the publication, SHP-1 | Homo sapiens | |
39 | - |
AAAAApYEEVH | pH 7.4, temperature not specified in the publication, RPTPalpha | Homo sapiens | |
41 | - |
RRISTpYAA | pH 7.4, temperature not specified in the publication, RPTPalpha | Homo sapiens | |
43 | - |
SASASpYSASA | pH 7.4, temperature not specified in the publication, RPTPalpha | Homo sapiens | |
69 | - |
WDEDFpYSASA | pH 7.4, temperature not specified in the publication, RPTPalpha | Homo sapiens | |
81 | - |
ARKRIpYAA | pH 7.4, temperature not specified in the publication, RPTPalpha | Homo sapiens |