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Literature summary for 3.1.3.48 extracted from

  • Gallo, G.; Ramos, T.C.; Tavares, F.; Rocha, A.A.; Machi, E.; Schenkman, S.; Bahia, D.; Pesquero, J.B.; Wuertele, M.
    Biochemical characterization of a protein tyrosine phosphatase from Trypanosoma cruzi involved in metacyclogenesis and cell invasion (2011), Biochem. Biophys. Res. Commun., 408, 427-431.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged enzyme expression in Escherichia coli Trypanosoma cruzi

Inhibitors

Inhibitors Comment Organism Structure
3-(3,5-dibromo-4-hydroxybenzoyl)-2-ethylbenzofuran-6-sulfonic acid-(4-(thiazol-2-yl-sulfamyl)-phenyl)-amide a PTP1b inhibitor, inhibit 50% tog the infectivity of Trypanosoma cruzi trypomastigotes Trypanosoma cruzi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten steady state kinetics with PTP pseudo-substrate 4-nitrophenyl phosphate Trypanosoma cruzi
4.5
-
4-nitrophenyl phosphate pH 7.5, 22°C, recombinant enzyme Trypanosoma cruzi

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi
-
-
-
Trypanosoma cruzi Brener
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Trypanosoma cruzi

Source Tissue

Source Tissue Comment Organism Textmining
epimastigote
-
Trypanosoma cruzi
-
trypomastigote
-
Trypanosoma cruzi
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Trypanosoma cruzi 4-nitrophenol + phosphate
-
?
4-nitrophenyl phosphate + H2O
-
Trypanosoma cruzi Brener 4-nitrophenol + phosphate
-
?

Synonyms

Synonyms Comment Organism
protein tyrosine phosphatase
-
Trypanosoma cruzi
TcPTP1
-
Trypanosoma cruzi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at room temperature Trypanosoma cruzi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8
-
4-nitrophenyl phosphate pH 7.5, 22°C, recombinant enzyme Trypanosoma cruzi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Trypanosoma cruzi

General Information

General Information Comment Organism
evolution Protein tyrosine phosphatases form a large family of enzymes involved in the regulation of numerous cellular functions in eukaryotes Trypanosoma cruzi