Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.48 extracted from

  • Chatterjee, D.; Mitra, A.; Levina, A.; Lay, P.A.
    A potential role for protein tyrosine phosphatase inhibition by a RuIII-edta complex (edta = ethylenediaminetetraacetate) in its biological activity (2008), Chem. Commun. (Camb. ), 2008, 2864-2866.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
C235R the mutation results in enhanced enzyme stability Yersinia enterocolitica

General Stability

General Stability Organism
DTT stabilizes the enzyme Yersinia enterocolitica

Inhibitors

Inhibitors Comment Organism Structure
additional information the inhibition mechanism of [RuIII(EDTA)(OH2/OH)]1-/2- shows similar interactions, that may be important in the anti-cancer properties of the active forms of many Ru pro-drugs Yersinia enterocolitica
pervanadate pervanadate inhibits PTP by irreversibly oxidizing the catalytic cysteine of PTP Yersinia enterocolitica
vanadate enzyme inhibition by vanadate takes place through an oxidant-independent pathway, the inhibition is reversible with EDTA Yersinia enterocolitica
[RuIII(EDTA)(OH2/OH)]1-/2- RuIII-EDTA inhibits PTP, like vanadate, through an oxidant-independent pathway. It inhibits PTP at physiological pH values by a mechanism that involves binding of the Cys residue of the catalytic domain of the enzyme, overview. At pH 7.4, the Ru-EDTA complex exists as a mixture of aqua and hydroxo-species. The inhibition is reversible or inhibited by glutathione Yersinia enterocolitica

Organism

Organism UniProt Comment Textmining
Yersinia enterocolitica
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
Yersinia enterocolitica
-

Storage Stability

Storage Stability Organism
-70°C, 2-3 months, loss of activity, DTT stabilizes Yersinia enterocolitica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme contains a Cys(X5)Arg catalytic domain Yersinia enterocolitica ?
-
?

Synonyms

Synonyms Comment Organism
protein tyrosine phosphatase
-
Yersinia enterocolitica
PTP
-
Yersinia enterocolitica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Yersinia enterocolitica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Yersinia enterocolitica

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information Ru(III) complexes with thiolato groups, stopped-flow kinetic studies with both RuIII-EDTA and RuIII-hEDTRA with the thiol-containing biomolecules, GSH or Cys, in the buffer Yersinia enterocolitica