Protein Variants | Comment | Organism |
---|---|---|
C235R | the mutation results in enhanced enzyme stability | Yersinia enterocolitica |
General Stability | Organism |
---|---|
DTT stabilizes the enzyme | Yersinia enterocolitica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the inhibition mechanism of [RuIII(EDTA)(OH2/OH)]1-/2- shows similar interactions, that may be important in the anti-cancer properties of the active forms of many Ru pro-drugs | Yersinia enterocolitica | |
pervanadate | pervanadate inhibits PTP by irreversibly oxidizing the catalytic cysteine of PTP | Yersinia enterocolitica | |
vanadate | enzyme inhibition by vanadate takes place through an oxidant-independent pathway, the inhibition is reversible with EDTA | Yersinia enterocolitica | |
[RuIII(EDTA)(OH2/OH)]1-/2- | RuIII-EDTA inhibits PTP, like vanadate, through an oxidant-independent pathway. It inhibits PTP at physiological pH values by a mechanism that involves binding of the Cys residue of the catalytic domain of the enzyme, overview. At pH 7.4, the Ru-EDTA complex exists as a mixture of aqua and hydroxo-species. The inhibition is reversible or inhibited by glutathione | Yersinia enterocolitica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yersinia enterocolitica | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Yersinia enterocolitica | - |
Storage Stability | Organism |
---|---|
-70°C, 2-3 months, loss of activity, DTT stabilizes | Yersinia enterocolitica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme contains a Cys(X5)Arg catalytic domain | Yersinia enterocolitica | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
protein tyrosine phosphatase | - |
Yersinia enterocolitica |
PTP | - |
Yersinia enterocolitica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Yersinia enterocolitica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Yersinia enterocolitica |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Ru(III) complexes with thiolato groups, stopped-flow kinetic studies with both RuIII-EDTA and RuIII-hEDTRA with the thiol-containing biomolecules, GSH or Cys, in the buffer | Yersinia enterocolitica |