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Literature summary for 3.1.3.48 extracted from

  • Wang, Y.; Meng, F.; Zhang, Y.
    Expression, purification and characterization of recombinant protein tyrosine phosphatase from Thermus thermophilus HB27 (2009), Acta Biochim. Biophys. Sin. (Shanghai), 41, 689-698.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of the active and soluble PTPase in Escherichia coli strain BL21(DE3) Thermus thermophilus

Inhibitors

Inhibitors Comment Organism Structure
Cl-
-
Thermus thermophilus
Cu2+
-
Thermus thermophilus
SO42-
-
Thermus thermophilus
Zn2+
-
Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.01
-
4-nitrophenyl phosphate pH 3.8, 75°C, recombinant enzyme Thermus thermophilus
37.88
-
4-nitrophenyl phosphate pH 3.8, 30°C, recombinant enzyme Thermus thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ activates the recombinant enzyme Thermus thermophilus
Ca2+ activates the recombinant enzyme Thermus thermophilus
Mg2+ activates the recombinant enzyme Thermus thermophilus
Mn2+ activates 3fold at 5 mM, recombinant enzyme Thermus thermophilus
Ni2+ activates the recombinant enzyme Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
x * 22000, recombinant enzyme, SDS-PAGE, x * 22260, sequence calculation Thermus thermophilus
22260
-
x * 22000, recombinant enzyme, SDS-PAGE, x * 22260, sequence calculation Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q72JF6
-
-
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 Q72JF6
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant soluble PTPase 54.3fold from Escherichia coli strain BL21(DE3) by heat treatment and gel filtration to over 95% purity Thermus thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.02
-
purified recombinant enzyme Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Thermus thermophilus 4-nitrophenol + phosphate
-
?
4-nitrophenyl phosphate + H2O
-
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 4-nitrophenol + phosphate
-
?

Subunits

Subunits Comment Organism
? x * 22000, recombinant enzyme, SDS-PAGE, x * 22260, sequence calculation Thermus thermophilus

Synonyms

Synonyms Comment Organism
low-molecular-weight protein tyrosine phosphatase
-
Thermus thermophilus
PTPase
-
Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
recombinant enzyme Thermus thermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 90 over 50% of maximal activity at 50-80°C, temperature profile, overview Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
4 h, purified recombinant enzyme, no significant decrease in activity Thermus thermophilus
60
-
4 h, purified recombinant enzyme, no significant decrease in activity Thermus thermophilus
70
-
4 h, purified recombinant enzyme, loss of 34% activity Thermus thermophilus
80
-
1 h, purified recombinant enzyme, 30% activity remaining Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
670.9
-
4-nitrophenyl phosphate pH 3.8, 30°C, recombinant enzyme Thermus thermophilus
4013
-
4-nitrophenyl phosphate pH 3.8, 75°C, recombinant enzyme Thermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8 4.8 recombinant enzyme Thermus thermophilus

pH Range

pH Minimum pH Maximum Comment Organism
2.2 10 pH profile, overview Thermus thermophilus

General Information

General Information Comment Organism
physiological function the heat-resistant PTPase may play important roles in vivo in the adaptation of the microorganism to extreme temperatures and specific nutritional conditions Thermus thermophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
17.71
-
4-nitrophenyl phosphate pH 3.8, 30°C, recombinant enzyme Thermus thermophilus
667.8
-
4-nitrophenyl phosphate pH 3.8, 75°C, recombinant enzyme Thermus thermophilus