Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.48 extracted from

  • Tsuruta, H.; Mikami, B.; Yamamoto, C.; Yamagata, H.
    The role of group bulkiness in the catalytic activity of psychrophile cold-active protein tyrosine phosphatase (2008), FEBS J., 275, 4317-4328.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli AD494 (DE3) cells Shewanella sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method, using a reservoir solution containing 30% (w/v) polyethylene glycol 4000, 0.2 M ammonium acetate, and 0.1 M Tris-HCl (pH 8.5) Shewanella sp.

Protein Variants

Protein Variants Comment Organism
I115A no activity Shewanella sp.
I115F the mutant enzyme possesses a higher kcat at low temperatures compared to the wild type enzyme, the thermostability of the I115F mutant enzyme exhibits higher catalytic efficiency than the wild type enzyme Shewanella sp.
I115L the mutant enzyme possesses a kcat at low temperatures similar to the wild type enzyme Shewanella sp.
I115M the mutant enzyme possesses a higher kcat at low temperatures compared to the wild type enzyme, the thermostability of the I115M mutant enzyme exhibits higher catalytic efficiency than the wild type enzyme Shewanella sp.
I115V the mutant enzyme possesses a kcat at low temperatures similar to the wild type enzyme Shewanella sp.
I307A the mutant enzyme possesses a lower kcat at low temperatures compared to the wild type enzyme Shewanella sp.
I307F the mutant enzyme possesses a lower kcat at low temperatures compared to the wild type enzyme Shewanella sp.
I307L the mutant enzyme possesses a lower kcat at low temperatures compared to the wild type enzyme Shewanella sp.
I307M the mutant enzyme possesses a lower kcat at low temperatures compared to the wild type enzyme Shewanella sp.
L145A the mutant enzyme possesses a lower kcat at low temperatures compared to the wild type enzyme Shewanella sp.
L145F the mutant enzyme possesses a lower kcat at low temperatures compared to the wild type enzyme Shewanella sp.
L145M the mutant enzyme possesses a lower kcat at low temperatures compared to the wild type enzyme Shewanella sp.
L145V the mutant enzyme possesses a lower kcat at low temperatures compared to the wild type enzyme Shewanella sp.
V79A the mutant enzyme possesses a lower kcat at low temperatures compared to the wild type enzyme Shewanella sp.
V79I the mutant enzyme possesses a lower kcat at low temperatures compared to the wild type enzyme Shewanella sp.
V79L the mutant enzyme possesses a lower kcat at low temperatures compared to the wild type enzyme Shewanella sp.
V79M no activity Shewanella sp.
V85A no activity Shewanella sp.
V85F the mutant enzyme possesses a lower kcat at low temperatures compared to the wild type enzyme Shewanella sp.
V85I the mutant enzyme possesses a lower kcat at low temperatures compared to the wild type enzyme Shewanella sp.
V85L the mutant enzyme possesses a lower kcat at low temperatures compared to the wild type enzyme Shewanella sp.
V85M the mutant enzyme possesses a lower kcat at low temperatures compared to the wild type enzyme Shewanella sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.45
-
4-nitrophenyl phosphate mutant enzyme I115F, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C Shewanella sp.
0.46
-
4-nitrophenyl phosphate mutant enzyme I115L, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C Shewanella sp.
0.46
-
4-nitrophenyl phosphate mutant enzyme I115M, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C Shewanella sp.
0.48
-
4-nitrophenyl phosphate mutant enzyme V79I, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C Shewanella sp.
0.51
-
4-nitrophenyl phosphate mutant enzyme L145A, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C Shewanella sp.
0.55
-
4-nitrophenyl phosphate mutant enzyme V85F, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C Shewanella sp.
0.59
-
4-nitrophenyl phosphate mutant enzyme L145V, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C Shewanella sp.
0.6
-
4-nitrophenyl phosphate mutant enzyme I115V, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C Shewanella sp.
0.6
-
4-nitrophenyl phosphate mutant enzyme V85M, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C Shewanella sp.
0.62
-
4-nitrophenyl phosphate wild type enzyme, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C Shewanella sp.
0.66
-
4-nitrophenyl phosphate mutant enzyme V79L, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C Shewanella sp.
0.71
-
4-nitrophenyl phosphate mutant enzyme L145F, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C Shewanella sp.
0.8
-
4-nitrophenyl phosphate mutant enzyme V85L, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C Shewanella sp.
0.83
-
4-nitrophenyl phosphate mutant enzyme L145M, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C Shewanella sp.
2.4
-
4-nitrophenyl phosphate mutant enzyme I307L, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C Shewanella sp.
4.01
-
4-nitrophenyl phosphate mutant enzyme I307A, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C Shewanella sp.
5.03
-
4-nitrophenyl phosphate mutant enzyme V79A, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C Shewanella sp.
6
-
4-nitrophenyl phosphate mutant enzyme I307F, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C Shewanella sp.
6.3
-
4-nitrophenyl phosphate mutant enzyme I307M, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C Shewanella sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains 1.79 mol of Zn2+ per mole Shewanella sp.

Organism

Organism UniProt Comment Textmining
Shewanella sp. Q9S427
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography, cellulofine-HAP column chromatography, and Mono-Q column chromatography Shewanella sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Shewanella sp. 4-nitrophenol + phosphate
-
?

Synonyms

Synonyms Comment Organism
CAPTPase
-
Shewanella sp.
cold-active protein tyrosine phosphatase
-
Shewanella sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 40 during a 60 min incubation at 30°C, the activity of the I115M mutant enzyme gradually decreases to 62%, whereas the activity of the I115F mutant decreases to 38%, at 40°C the activities decrease to 48% for I115M and 10% for I115F, the wild type enzyme maintains 80% and 75% activity after incubation at 30°C and 40°C for 60 min Shewanella sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.33
-
4-nitrophenyl phosphate mutant enzyme V79A, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C Shewanella sp.
7.1
-
4-nitrophenyl phosphate mutant enzyme I307A, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C Shewanella sp.
11
-
4-nitrophenyl phosphate mutant enzyme I307F, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C Shewanella sp.
11.4
-
4-nitrophenyl phosphate mutant enzyme L145V, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C Shewanella sp.
21.9
-
4-nitrophenyl phosphate mutant enzyme L145A, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C Shewanella sp.
26
-
4-nitrophenyl phosphate mutant enzyme I307L, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C Shewanella sp.
36.9
-
4-nitrophenyl phosphate mutant enzyme I307M, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C Shewanella sp.
38.3
-
4-nitrophenyl phosphate mutant enzyme V85F, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C Shewanella sp.
40.7
-
4-nitrophenyl phosphate mutant enzyme V79L, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C Shewanella sp.
62.3
-
4-nitrophenyl phosphate mutant enzyme V85M, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C Shewanella sp.
66.2
-
4-nitrophenyl phosphate mutant enzyme V79I, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C Shewanella sp.
129
-
4-nitrophenyl phosphate mutant enzyme L145F, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C Shewanella sp.
129.7
-
4-nitrophenyl phosphate mutant enzyme V85L, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C Shewanella sp.
167.1
-
4-nitrophenyl phosphate mutant enzyme L145M, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C Shewanella sp.
184.1
-
4-nitrophenyl phosphate mutant enzyme I115L, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C Shewanella sp.
184.8
-
4-nitrophenyl phosphate mutant enzyme I115V, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C Shewanella sp.
184.9
-
4-nitrophenyl phosphate wild type enzyme, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C Shewanella sp.
223.7
-
4-nitrophenyl phosphate mutant enzyme I115M, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C Shewanella sp.
262
-
4-nitrophenyl phosphate mutant enzyme I115F, in 50 mM MES-NaOH (pH 6.0), 2.5 mM MgCl2, at 5°C Shewanella sp.