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Literature summary for 3.1.3.48 extracted from

  • Dahche, H.; Abdullah, A.; Ben Potters, M.; Kennelly, P.J.
    A PPM-family protein phosphatase from the thermoacidophile Thermoplasma volcanium hydrolyzes protein-bound phosphotyrosine (2009), Extremophiles, 13, 371-377.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells Thermoplasma volcanium

Inhibitors

Inhibitors Comment Organism Structure
Zn2+ Zn2+ inhibits the enzyme irreversibly in a concentration-dependent manner Thermoplasma volcanium

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ Mn2+ supports catalysis Thermoplasma volcanium
additional information the inclusion of Mg2+, Ni2+, Ca2+, and Cu2+ along with Mn2+ has little effect on activity relative to that observed with Mn2+ alone Thermoplasma volcanium

Organism

Organism UniProt Comment Textmining
Thermoplasma volcanium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
S-protein agarose chromatography Thermoplasma volcanium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Thermoplasma volcanium p-nitrophenol + phosphate
-
?
additional information PPM displays little activity toward P-Ser/Thr histones Thermoplasma volcanium ?
-
?
phosphotyrosyl casein + H2O
-
Thermoplasma volcanium tyrosylcasein + phosphate
-
?
phosphotyrosyl myelin basic protein + H2O
-
Thermoplasma volcanium tyrosyl-myelin basic protein + phosphate
-
?
phosphotyrosyl reduced carboxyamidomethylated and maleylated lysozyme + H2O
-
Thermoplasma volcanium tyrosyl-reduced carboxyamidomethylated and maleylated lysozyme + phosphate
-
?

Synonyms

Synonyms Comment Organism
PPM the recombinant protein exhibits protein-tyrosine phosphatase in addition to its predicted protein-serine/threonine phosphatase activity Thermoplasma volcanium