Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells | Thermoplasma volcanium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | Zn2+ inhibits the enzyme irreversibly in a concentration-dependent manner | Thermoplasma volcanium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | Mn2+ supports catalysis | Thermoplasma volcanium | |
additional information | the inclusion of Mg2+, Ni2+, Ca2+, and Cu2+ along with Mn2+ has little effect on activity relative to that observed with Mn2+ alone | Thermoplasma volcanium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoplasma volcanium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
S-protein agarose chromatography | Thermoplasma volcanium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Thermoplasma volcanium | p-nitrophenol + phosphate | - |
? | |
additional information | PPM displays little activity toward P-Ser/Thr histones | Thermoplasma volcanium | ? | - |
? | |
phosphotyrosyl casein + H2O | - |
Thermoplasma volcanium | tyrosylcasein + phosphate | - |
? | |
phosphotyrosyl myelin basic protein + H2O | - |
Thermoplasma volcanium | tyrosyl-myelin basic protein + phosphate | - |
? | |
phosphotyrosyl reduced carboxyamidomethylated and maleylated lysozyme + H2O | - |
Thermoplasma volcanium | tyrosyl-reduced carboxyamidomethylated and maleylated lysozyme + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PPM | the recombinant protein exhibits protein-tyrosine phosphatase in addition to its predicted protein-serine/threonine phosphatase activity | Thermoplasma volcanium |