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Literature summary for 3.1.3.48 extracted from

  • Braithwaite, S.P.; Xu, J.; Leung, J.; Urfer, R.; Nikolich, K.; Oksenberg, D.; Lombroso, P.J.; Shamloo, M.
    Expression and function of striatal enriched protein tyrosine phosphatase is profoundly altered in cerebral ischemia (2008), Eur. J. Neurosci., 27, 2444-2452.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
STEP46 is expressed in Escherichia coli Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm isoforms STEP46 and STEP33 are found predominantly in the cytoplasmic fraction Rattus norvegicus 5737
-
membrane the majority of STEP61 is found in membrane-bound compartments Rattus norvegicus 16020
-
soluble there is a significant processing of STEP after ischemia to generate a novel species, STEP33, resulting in a redistribution of STEP from membrane-bound to soluble compartments Rattus norvegicus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
low molecular-weight isoform STEP33, SDS-PAGE Rattus norvegicus
46000
-
recombinant STEP46, SDS-PAGE Rattus norvegicus
61000
-
STEP61, SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione Sepharose beads chromatography Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain in global cerebral ischemia STEP mRNA is selectively down-regulated in brain areas susceptible to ischemic damage, while in regions of the brain that are relatively resistant to ischemic damage STEP mRNA levels are increased Rattus norvegicus
-
dentate gyrus
-
Rattus norvegicus
-
hippocampus
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Rattus norvegicus 4-nitrophenol + phosphate
-
?
phosphorylated ERK + H2O the cleavage of STEP leads to a catalytically active form, but this cleaved form no longer binds to and dephosphorylates its substrate phosphorylated ERK Rattus norvegicus ERK + phosphate
-
?

Synonyms

Synonyms Comment Organism
STEP
-
Rattus norvegicus
STEP33 STEP is processed into a 33 kDa isoform by calpain-mediated cleavage, STEP33 retains catalytic activity but is unable to bind to phosphorylated ERK Rattus norvegicus
STEP46 recombinant enzyme Rattus norvegicus
STEP61 STEP61 is the normally expressed enzyme form Rattus norvegicus
striatal enriched protein tyrosine phosphatase
-
Rattus norvegicus