Cloned (Comment) | Organism |
---|---|
STEP46 is expressed in Escherichia coli | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | isoforms STEP46 and STEP33 are found predominantly in the cytoplasmic fraction | Rattus norvegicus | 5737 | - |
membrane | the majority of STEP61 is found in membrane-bound compartments | Rattus norvegicus | 16020 | - |
soluble | there is a significant processing of STEP after ischemia to generate a novel species, STEP33, resulting in a redistribution of STEP from membrane-bound to soluble compartments | Rattus norvegicus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
low molecular-weight isoform STEP33, SDS-PAGE | Rattus norvegicus |
46000 | - |
recombinant STEP46, SDS-PAGE | Rattus norvegicus |
61000 | - |
STEP61, SDS-PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
glutathione Sepharose beads chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | in global cerebral ischemia STEP mRNA is selectively down-regulated in brain areas susceptible to ischemic damage, while in regions of the brain that are relatively resistant to ischemic damage STEP mRNA levels are increased | Rattus norvegicus | - |
dentate gyrus | - |
Rattus norvegicus | - |
hippocampus | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Rattus norvegicus | 4-nitrophenol + phosphate | - |
? | |
phosphorylated ERK + H2O | the cleavage of STEP leads to a catalytically active form, but this cleaved form no longer binds to and dephosphorylates its substrate phosphorylated ERK | Rattus norvegicus | ERK + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
STEP | - |
Rattus norvegicus |
STEP33 | STEP is processed into a 33 kDa isoform by calpain-mediated cleavage, STEP33 retains catalytic activity but is unable to bind to phosphorylated ERK | Rattus norvegicus |
STEP46 | recombinant enzyme | Rattus norvegicus |
STEP61 | STEP61 is the normally expressed enzyme form | Rattus norvegicus |
striatal enriched protein tyrosine phosphatase | - |
Rattus norvegicus |