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Literature summary for 3.1.3.48 extracted from

  • Chen, Y.; Xu, Y.; Bao, Q.; Xing, Y.; Li, Z.; Lin, Z.; Stock, J.B.; Jeffrey, P.D.; Shi, Y.
    Structural and biochemical insights into the regulation of protein phosphatase 2A by small t antigen of SV40 (2007), Nat. Struct. Mol. Biol., 14, 527-534.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in insect cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method with 0.2 M MgCl2, 4.5% PEG 10000 (w/v) and 0.1 M HEPES (pH 7.5) Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
small t antigen small t antigen of DNA tumor virus SV40 inhibits the phosphatase activity of the PP2A core enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0024
-
p-nitrophenyl phosphate
-
Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione affinity column chromatography, Source 15Q column chromatography, and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl phosphate + H2O
-
Homo sapiens p-nitrophenol + phosphate
-
?

Synonyms

Synonyms Comment Organism
PP2A
-
Homo sapiens
protein phosphatase 2A
-
Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.002
-
using p-nitrophenyl phosphate as a substrate Homo sapiens small t antigen