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Literature summary for 3.1.3.48 extracted from

  • Picha, K.M.; Patel, S.S.; Mandiyan, S.; Koehn, J.; Wennogle, L.P.
    The role of the C-terminal domain of protein tyrosine phosphatase-1B in phosphatase activity and substrate binding (2007), J. Biol. Chem., 282, 2911-2917.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21 (DE3) Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0096
-
GNGDpYMPMSPKS reaction catalyzed by the full-length enzyme in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, and 3 mM dithiothreitol, at 25°C Homo sapiens
0.0191
-
GNGDpYMPMSPKS reaction catalyzed by the catalyctic domain of the enzyme in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, and 3 mM dithiothreitol, at 25°C Homo sapiens
1.7
-
p-nitrophenyl phosphate reaction catalyzed by the full-length enzyme in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, and 3 mM dithiothreitol, at 25°C Homo sapiens
2.7
-
p-nitrophenyl phosphate reaction catalyzed by the catalyctic domain of the enzyme in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, and 3 mM dithiothreitol, at 25°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
POROS 20SP column chromatography and Source 30Q column chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
hippocampus
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GNGDpYMPMSPKS + H2O
-
Homo sapiens GNGDYMPMSPKS + phosphate
-
?
p-nitrophenyl phosphate + H2O
-
Homo sapiens p-nitrophenol + phosphate
-
?

Synonyms

Synonyms Comment Organism
protein tyrosine phosphatase-1B
-
Homo sapiens
PTP-1B
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.9
-
p-nitrophenyl phosphate reaction catalyzed by the full-length enzyme in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, and 3 mM dithiothreitol, at 25°C Homo sapiens
3.1
-
p-nitrophenyl phosphate reaction catalyzed by the catalyctic domain of the enzyme in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, and 3 mM dithiothreitol, at 25°C Homo sapiens
7.7
-
GNGDpYMPMSPKS reaction catalyzed by the full-length enzyme in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, and 3 mM dithiothreitol, at 25°C Homo sapiens
18.4
-
GNGDpYMPMSPKS reaction catalyzed by the catalyctic domain of the enzyme in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, and 3 mM dithiothreitol, at 25°C Homo sapiens