Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21 (DE3) | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0096 | - |
GNGDpYMPMSPKS | reaction catalyzed by the full-length enzyme in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, and 3 mM dithiothreitol, at 25°C | Homo sapiens | |
0.0191 | - |
GNGDpYMPMSPKS | reaction catalyzed by the catalyctic domain of the enzyme in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, and 3 mM dithiothreitol, at 25°C | Homo sapiens | |
1.7 | - |
p-nitrophenyl phosphate | reaction catalyzed by the full-length enzyme in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, and 3 mM dithiothreitol, at 25°C | Homo sapiens | |
2.7 | - |
p-nitrophenyl phosphate | reaction catalyzed by the catalyctic domain of the enzyme in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, and 3 mM dithiothreitol, at 25°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
POROS 20SP column chromatography and Source 30Q column chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hippocampus | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GNGDpYMPMSPKS + H2O | - |
Homo sapiens | GNGDYMPMSPKS + phosphate | - |
? | |
p-nitrophenyl phosphate + H2O | - |
Homo sapiens | p-nitrophenol + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
protein tyrosine phosphatase-1B | - |
Homo sapiens |
PTP-1B | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.9 | - |
p-nitrophenyl phosphate | reaction catalyzed by the full-length enzyme in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, and 3 mM dithiothreitol, at 25°C | Homo sapiens | |
3.1 | - |
p-nitrophenyl phosphate | reaction catalyzed by the catalyctic domain of the enzyme in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, and 3 mM dithiothreitol, at 25°C | Homo sapiens | |
7.7 | - |
GNGDpYMPMSPKS | reaction catalyzed by the full-length enzyme in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, and 3 mM dithiothreitol, at 25°C | Homo sapiens | |
18.4 | - |
GNGDpYMPMSPKS | reaction catalyzed by the catalyctic domain of the enzyme in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, and 3 mM dithiothreitol, at 25°C | Homo sapiens |