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Literature summary for 3.1.3.48 extracted from

  • Sohn, J.; Buhrman, G.; Rudolph, J.
    Kinetic and structural studies of specific protein-protein interactions in substrate catalysis by Cdc25B phosphatase (2007), Biochemistry, 46, 807-818.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Homo sapiens

Protein Variants

Protein Variants Comment Organism
C473D active site mutant, weak substrate affinity and dissociates rapidly Homo sapiens
C473S strong substrate affinity and dissociates slowly Homo sapiens
R492L hotspot mutant, dramatically decreased activity despite showing no significant changes in crystal structure Homo sapiens
Y497A shows a significantly slower rate constant for association compared to that of the wild type enzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P30305
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphorylated Cdk2-pTpY/CycA protein + H2O
-
Homo sapiens Cdk2-pTpY/CycA protein + phosphate
-
?
phosphotyrosine + H2O
-
Homo sapiens tyrosine + phosphate
-
?

Synonyms

Synonyms Comment Organism
Cdc25B phosphatase
-
Homo sapiens
dual-specificity phosphatase
-
Homo sapiens