Protein Variants | Comment | Organism |
---|---|---|
D415A | isoform SynPPM3, no activity with 4-nitrophenyl phosphate, reduction in phosphoserine- and phosphotyrosine-activity against casein and carboxyamidomethylated and maleylated lysozyme | Synechocystis sp. |
D415N | isoform SynPPM3, 5% activity with 4-nitrophenyl phosphate compared to wild-type, 50% reduction in phosphotyrosine-activity against carboxyamidomethylated and maleylated lysozyme | Synechocystis sp. |
D444N | isoform SynPPM3, no activity with 4-nitrophenyl phosphate, reduction in phosphoserine- and phosphotyrosine-activity against casein and carboxyamidomethylated and maleylated lysozyme | Synechocystis sp. |
D604N | isoform SynPPM3, no activity | Synechocystis sp. |
D608N | isoform SynPPM3, 50% activity with 4-nitrophenyl phosphate compared to wild-type, 4fold increase in phosphotyrosine-activity against carboxyamidomethylated and maleylated lysozyme | Synechocystis sp. |
D648N | isoform SynPPM3, 5% activity with 4-nitrophenyl phosphate compared to wild-type, large reduction in phosphoserine- and phosphotyrosine-activity against casein and carboxyamidomethylated and maleylated lysozyme | Synechocystis sp. |
E414Q | isoform SynPPM3, similar activity with 4-nitrophenyl phosphate as wild-type, but 50% reduction in phosphoserine- and phosphotyrosine-activity against casein and carboxyamidomethylated and maleylated lysozyme | Synechocystis sp. |
E618Q | isoform SynPPM3, 87% of activity with 4-nitrophenyl phosphate compared to wild-type, reduction in phosphoserine- and phosphotyrosine-activity against casein and carboxyamidomethylated and maleylated lysozyme | Synechocystis sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaF | moderate inhibition, both SynPPP1 and SYnPPM3 | Synechocystis sp. | |
o-vanadate | both SynPPP1 and SYnPPM3 | Synechocystis sp. | |
okadaic acid | both SynPPP1 and SYnPPM3 | Synechocystis sp. | |
Sodium diphosphate | both isoforms, inhibition by millimolar concentrations | Synechocystis sp. | |
Tartrate | both SynPPP1 and SYnPPM3 | Synechocystis sp. | |
tetramisole | both SynPPP1 and SYnPPM3 | Synechocystis sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00044 | - |
casein | isoform SynPPM3, activity against phosphoserine residues, pH 7.5, 37°C | Synechocystis sp. | |
0.00045 | - |
carboxyamidomethylated and maleylated lysozyme | isoform SynPPM3, activity against phosphotyrosine residues, pH 7.5, 37°C | Synechocystis sp. | |
0.0017 | - |
carboxyamidomethylated and maleylated lysozyme | isoform SynPPP1, activity against phosphoserine residues, pH 7.5, 37°C | Synechocystis sp. | |
0.0063 | - |
myelin basic protein | isoform SynPPM3, activity against phosphoserine residues, pH 7.5, 37°C | Synechocystis sp. | |
0.022 | - |
myelin basic protein | isoform SynPPP1, activity against phosphoserine residues, pH 7.5, 37°C | Synechocystis sp. | |
12.7 | - |
4-nitrophenyl phosphate | isoform SynPPM3, pH 7.5, 37°C | Synechocystis sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. | - |
strain PCC 6803, isoforms SynPPP1 and SynPPM3 both acting on phosphotyrosine- as well as phosphoserine-containing proteins | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.066 | - |
isoform SynPPM3, pH 7.5, 37°C | Synechocystis sp. |
0.084 | - |
isoform SynPPP1, pH 7.5, 37°C | Synechocystis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Synechocystis sp. | 4-nitrophenol + phosphate | - |
? | |
carboxyamidomethylated and maleylated lysozyme + H2O | - |
Synechocystis sp. | ? | - |
? | |
casein + H2O | - |
Synechocystis sp. | ? | - |
? | |
myelin basic protein + H2O | - |
Synechocystis sp. | ? | - |
? |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | isoform SynPPM3 | Synechocystis sp. |
7.5 | 8.4 | isoform SynPPP1 | Synechocystis sp. |