Protein Variants | Comment | Organism |
---|---|---|
D112N | GST fusion protein, no enzymic activity | Shewanella sp. |
D115N | GST fusion protein, no enzymic activity | Shewanella sp. |
D76N | GST fusion protein, no enzymic activity | Shewanella sp. |
H148N | GST fusion protein, no enzymic activity | Shewanella sp. |
H44N | GST fusion protein, acitivity slightly lower than wild-type | Shewanella sp. |
H78N | GST fusion protein, 8fold reduction of activity compared to wild-type | Shewanella sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.53 | - |
4-nitrophenyl phosphate | mutant H44N, GST fusion protein | Shewanella sp. | |
0.58 | - |
4-nitrophenyl phosphate | wild-type, GST fusion protein | Shewanella sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Shewanella sp. | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
[a protein]-tyrosine phosphate + H2O = [a protein]-tyrosine + phosphate | H148 acts as a general acid catalyst, D115 assists the protonation of the leaving group of a substrate, and D76 and D112 are involved in binding of magnesium ions | Shewanella sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Shewanella sp. | 4-nitrophenol + phosphate | - |
? |