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Literature summary for 3.1.3.48 extracted from

  • Welte, S.; Baringhaus, K.H.; Schmider, W.; Mueller, G.; Petry, S.; Tennagels, N.
    6,8-Difluoro-4-methylumbiliferyl phosphate: a fluorogenic substrate for protein tyrosine phosphatases (2005), Anal. Biochem., 338, 32-38.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1-ethyl-6-methyl-3-phenyl-1H-pyrimido(5,4-e)(1,2,4)triazine-5,7-dione covalent mode of action Homo sapiens
1-ethyl-6-methyl-3-phenyl-1H-pyrimido(5,4-e)(1,2,4)triazine-5,7-dione covalent mode of action Yersinia sp.
2,2-dioxo-2,3-dihydro-2-OMEGA-16-benzo (1,2,3)oxathiazole-6-carboxylic acid (5-phenylsulfanyl-1H-benzoimidazol-2-ylmethyl)-amide competitive, noncovalent mode of action Homo sapiens
2,2-dioxo-2,3-dihydro-2-OMEGA-16-benzo (1,2,3)oxathiazole-6-carboxylic acid (5-phenylsulfanyl-1H-benzoimidazol-2-ylmethyl)-amide competitive, noncovalent mode of action Yersinia sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0029
-
insulin receptor phosphopeptide isoform PTP1B, 37°C, pH 6.9 Homo sapiens
0.02
-
4,6,8-trimethyl-2-oxo-2H-chromen-7-yl dihydrogen phosphate isoform PTP1B, 37°C, pH 6.9 Homo sapiens
0.115
-
4,6,8-trimethyl-2-oxo-2H-chromen-7-yl dihydrogen phosphate 37°C, pH 6.9 Yersinia sp.
0.156
-
4,6,8-trimethyl-2-oxo-2H-chromen-7-yl dihydrogen phosphate isoform CD45, 37°C, pH 6.9 Homo sapiens
1.4
-
4-nitrophenyl phosphate isoform PTP1B, 37°C, pH 6.9 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
isoforms PTP1B, CD45, comercial preparations
-
Yersinia sp.
-
isoform YOP, comercial preparation
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
continuous and highly specific assay method based on substrate 6,8-difluoro-4-methylumbiliferyl phosphate Homo sapiens
additional information
-
continuous and highly specific assay method based on substrate 6,8-difluoro-4-methylumbiliferyl phosphate Yersinia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Homo sapiens 4-nitrophenol + phosphate
-
?
4-nitrophenyl phosphate + H2O
-
Yersinia sp. 4-nitrophenol + phosphate
-
?
6,8-difluoro-4-methylumbelliferyl phosphate + H2O
-
Homo sapiens 6,8-difluoro-4-methylumbelliferone + phosphate
-
?
6,8-difluoro-4-methylumbelliferyl phosphate + H2O
-
Yersinia sp. 6,8-difluoro-4-methylumbelliferone + phosphate
-
?
insulin receptor phosphopeptide + H2O
-
Homo sapiens insulin receptor peptide + phosphate
-
?
insulin receptor phosphopeptide + H2O
-
Yersinia sp. insulin receptor peptide + phosphate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2
-
4,6,8-trimethyl-2-oxo-2H-chromen-7-yl dihydrogen phosphate isoform CD45, 37°C, pH 6.9 Homo sapiens
16.8
-
4,6,8-trimethyl-2-oxo-2H-chromen-7-yl dihydrogen phosphate isoform PTP1B, 37°C, pH 6.9 Homo sapiens
27.5
-
4-nitrophenyl phosphate isoform PTP1B, 37°C, pH 6.9 Homo sapiens
36.7
-
4,6,8-trimethyl-2-oxo-2H-chromen-7-yl dihydrogen phosphate 37°C, pH 6.9 Yersinia sp.
61.5
-
insulin receptor phosphopeptide isoform PTP1B, 37°C, pH 6.9 Homo sapiens
71.6
-
4,6,8-trimethyl-2-oxo-2H-chromen-7-yl dihydrogen phosphate isoform CD45, 37°C, pH 6.9 Homo sapiens