Activating Compound | Comment | Organism | Structure |
---|---|---|---|
1,2-epoxy-3-(p-nitrophenoxy)propane | C215D mutant, not wild type | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
wild type and C215D mutant, both are structurally identical | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C215D | active-site mutant, similar Km-values as wild type, but reduced kcat-values and activation by 1,2-epoxy-3-(p-nitrophenoxy)propane, use as substrate-trapping mutant | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
p-nitrophenyl phosphate | 22°C, pH 6.0, mutant C215D | Homo sapiens | |
1.4 | - |
p-nitrophenyl phosphate | 22°C, pH 6.0, wild type | Homo sapiens | |
5.4 | - |
p-nitrophenyl phosphate | 22°C, pH 4.5, mutant C215D | Homo sapiens | |
5.8 | - |
p-nitrophenyl phosphate | 22°C, pH 4.5, wild type | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P18031 | isoform PTP1B | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
p-nitrophenyl phosphate + H2O | - |
Homo sapiens | p-nitrophenol + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PTP-1B | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
mutant C215D | Homo sapiens |
6 | - |
wild type | Homo sapiens |