Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.48 extracted from

  • Xie, L.; Zhang, Y.L.; Zhang, Z.Y.
    Design and characterization of an improved protein tyrosine phosphatase substrate-trapping mutant (2002), Biochemistry, 41, 4032-4039.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D181A substrate-trapping mutant, kinetic and thermodynamic characterization Homo sapiens
D181A/Q262A substrate-trapping mutant, kinetic and thermodynamic characterization, very high affinity for epidermal growth factor receptor Homo sapiens
additional information design of further substrate-trapping mutants Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00035
-
DADEpYLIPQQG pH 7.0, 30°C, mutant Q262A Homo sapiens
0.0026
-
DADEpYLIPQQG pH 7.0, 25°C, wild type Homo sapiens
0.051
-
DADEpYLIPQQG pH 7.0, 30°C, mutant D181A/Q262A Homo sapiens
0.056
-
DADEpYLIPQQG pH 7.0, 25°C, mutant D181A Homo sapiens
0.058
-
p-nitrophenyl phosphate pH 7.0, 30°C, mutant D181A/Q262A Homo sapiens
0.08
-
p-nitrophenyl phosphate pH 7.0, 25°C, mutant D181A Homo sapiens
2.4
-
p-nitrophenyl phosphate pH 7.0, 25°C, wild type Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
isozyme PTP1B
-

Reaction

Reaction Comment Organism Reaction ID
[a protein]-tyrosine phosphate + H2O = [a protein]-tyrosine + phosphate thermodynamic parameters Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DADEpYLIPQQG + H2O
-
Homo sapiens DADEYLIPQQG + phosphate
-
?
p-nitrophenyl phosphate + H2O
-
Homo sapiens p-nitrophenol + phosphate
-
?