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Literature summary for 3.1.3.48 extracted from

  • Romsicki, Y.; Kennedy, B.P.; Asante-Appiah, E.
    Purification and characterization of T cell protein tyrosine phosphatase reveals significant functional homology to protein tyrosine phosphatase-1B (2003), Arch. Biochem. Biophys., 414, 40-50.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
N-benzoyl-L-glutamyl-[4-phosphono(difluoromethyl)]-L-phenylalanineamide i.e. BzN-EJJ-amide, 50% inhibition at 4 nM Homo sapiens
NaCl increase in Km-value with increase of ionic strength, 10-fold increase of Km-value from 0 to 200 mM NaCl Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
fluorescein diphosphate 22°C, pH 5.5 Homo sapiens
1.1
-
4-nitrophenyl phosphate 22°C, pH 5.5 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38500
-
1 * 38500, deduced from gene sequence Homo sapiens
40000
-
gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
recombinant enzyme
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein expressed Escherichia coli Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Homo sapiens 4-nitrophenol + phosphate
-
?
fluorescein diphosphate + H2O
-
Homo sapiens fluorescein phosphate + phosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 38500, deduced from gene sequence Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
42
-
above, significant denaturation, 40% w/v sucrose protects Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Homo sapiens