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Literature summary for 3.1.3.46 extracted from
- Characterization of phosphofructokinase 2 and of enzymes involved in the degradation of fructose 2,6-bisphosphate in yeast (1988), Eur. J. Biochem., 171, 599-608.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| benzoate | - |
Saccharomyces cerevisiae |  |
| salicylate | - |
Saccharomyces cerevisiae | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| fructose 6-phosphate | - |
Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0001 | - |
fructose 2,6-bisphosphate | - |
Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
2 * 56000 | Saccharomyces cerevisiae |
| 110000 | - |
gel filtration | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| fructose 2,6-bisphosphate + H2O | Saccharomyces cerevisiae | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 300fold | Saccharomyces cerevisiae |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate | bifunctional enzyme: 6-phosphofructo-2-kinase-fructose-2,6-bisphosphatase | Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, 10% glycerol, more than 1 week | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| fructose 2,6-bisphosphate + H2O | - |
Saccharomyces cerevisiae | fructose 6-phosphate + phosphate | - |
? | |
| fructose 2,6-bisphosphate + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 56000 | Saccharomyces cerevisiae |
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