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Literature summary for 3.1.3.43 extracted from
- Purification and characterization of pyruvate-dehydrogenase phosphatase from pig-heart muscle (1972), Eur. J. Biochem., 26, 96-105.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EGTA | - |
Sus scrofa |  |
| F- | 50% inhibition at 0.6 mM in the presence of Mg2+ | Sus scrofa | |
| KCl | inhibits reactivation | Sus scrofa | |
| N-ethylmaleimide | slight inhibition | Sus scrofa | |
| NaCl | inhibits reactivation | Sus scrofa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0076 | - |
[Pyruvate dehydrogenase (lipoamide)]-phosphate | - |
Sus scrofa |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Sus scrofa | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | overcomes EGTA inhibition | Sus scrofa | |
| Mg2+ | - |
Sus scrofa | |
| Mn2+ | - |
Sus scrofa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 92000 | 95000 | gel electrophoresis, SDS-PAGE | Sus scrofa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| [pyruvate dehydrogenase (lipoamide)]-phosphate + H2O | Sus scrofa | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | - |
Sus scrofa | - |
| muscle | - |
Sus scrofa | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -35°C, 4 weeks | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| [pyruvate dehydrogenase (lipoamide)]-phosphate + H2O | - |
Sus scrofa | [pyruvate dehydrogenase (lipoamide)] + phosphate | - |
ir | |
| [pyruvate dehydrogenase (lipoamide)]-phosphate + H2O | - |
Sus scrofa | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 2 | 4 | 40% loss of activity within 8 days, 80% within 19 days | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Sus scrofa |
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Release 2023.1 (January 2023)
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