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Literature summary for 3.1.3.4 extracted from

  • Cao, H.; Sethumadhavan, K.; Grimm, C.C.; Ullah, A.H.
    Characterization of a soluble phosphatidic acid phosphatase in bitter melon (Momordica charantia) (2014), PLoS ONE, 9, e106403.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
CuSO4
-
Momordica charantia
FeSO4
-
Momordica charantia
additional information presence of EDTA or EGTA does not significantly affect enzyme activity Momordica charantia
Na3VO4 2 mM, about 90% inhibition Momordica charantia
NaF
-
Momordica charantia
Triton X-100
-
Momordica charantia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5954
-
1,2-diacyl-sn-glycerol-3-phosphate pH 6.5, 53°C Momordica charantia

Metals/Ions

Metals/Ions Comment Organism Structure
Co(NO3)2 0.3 mM, about 3fold stimulation Momordica charantia
MnSO4 0.3 mM, about 3fold stimulation Momordica charantia
additional information activity is independent of Mg2+ Momordica charantia
ZnSO4 0.3 mM, about 2.5fold stimulation Momordica charantia

Organism

Organism UniProt Comment Textmining
Momordica charantia
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Momordica charantia

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon developing cotyledon Momordica charantia
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
312
-
pH 6.5, 53°C Momordica charantia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-diacyl-sn-glycerol-3-phosphate + H2O
-
Momordica charantia 1,2-diacyl-sn-glycerol + phosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
53 60
-
Momordica charantia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Momordica charantia