Cloned (Comment) | Organism |
---|---|
coding sequence of lipin 1b subcloned downstream of the GST tag in the pEBG expression vector, transfected into 293T cells. Adenoviral constructs for HA-tagged lipin 1b | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
D712E | lacks enzymatic activity, does not affect the interaction of lipin 1 and PPARalpha, but eliminates the ability of lipin 1 to interact with nuclear factor of activated T-cells c4. Also fails to repress nuclear factor of activated T-cells c4 transcriptional activity | Mus musculus |
I706F | lacks enzymatic activity, retains the ability to interact with and repress nuclear factor of activated T-cells c4 transcriptional activity | Mus musculus |
I707F | lacks enzymatic activity, retains the ability to interact with and repress nuclear factor of activated T-cells c4 transcriptional activity | Mus musculus |
I723F | lacks enzymatic activity, is unable to interact with nuclear factor of activated T-cells c4 and has no repressive effect on reporter gene activity | Mus musculus |
L724F | lacks enzymatic activity, is unable to interact with nuclear factor of activated T-cells c4 and has no repressive effect on reporter gene activity | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | lipin 1-mediated repression of nuclear factor of activated T-cells transcriptional activity occurs after nuclear factor of activated T-cells mobilization and activation by calcium | Mus musculus | |
Mg2+ | dependent on | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
3T3-L1 cell | - |
Mus musculus | - |
adipocyte | - |
Mus musculus | - |
preadipocyte | - |
Mus musculus | - |
Synonyms | Comment | Organism |
---|---|---|
lipin 1 | - |
Mus musculus |
Mg2+-dependent phosphatidic acid phosphatase | - |
Mus musculus |
PAP | - |
Mus musculus |
Organism | Comment | Expression |
---|---|---|
Mus musculus | lipin 1 knockdown by shRNA, decline in lipin 1 expression with increasing obesity in ob/ob mice | down |
General Information | Comment | Organism |
---|---|---|
malfunction | in fat pads from mice deficient for lipin 1 (fld mice) and in 3T3-L1 adipocytes depleted of lipin 1 there is increased expression of several nuclear factor of activated T-cells target genes including TNFalpha, resistin, FABP4 and PPARgamma. Lipin 1 with the highly conserved amino-terminal NLIP domain deleted (DELTAN) is capable of both interaction and repression | Mus musculus |
physiological function | lipin 1 is a potentially important link between triacylglycerol synthesis and adipose tissue inflammation. Lipin 1 represses nuclear factor of activated T-cells c4 transcriptional activity through protein-protein interaction and in the context of at least two different composite elements. Specific residues required for interaction with lipin 1 are contained within the RHD/DNA binding domain and carboxy terminus of nuclear factor of activated T-cells c4. Lipin 1 is present at the promoters of nuclear factor of activated T-cells c4 transcriptional targets in vivo. Lipin 1 protein and total PAP activity are decreased with increasing adiposity in the visceral, but not subcutaneous, fat pads of ob/ob mice. Lipin 1 can act to repress or activate transcription factors. Lipin 1 interacts with nuclear factor of activated T-cells c4 bound to DNA and is present at the promoters of nuclear factor of activated T-cells target genes. Lipin 1 recruits a histone deacetylase to repress nuclear factor of activated T-cells c4 transcription | Mus musculus |