Application | Comment | Organism |
---|---|---|
drug development | the PAP1 colorimetric assay with pure enzyme and a water-soluble phosphatidate substrate is applicability to a 96-well format, which may facilitate a large-scale screen of PAP1 inhibitors (or activators) | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DMSO | addition to the reaction mixture results in a dose-dependent inhibition of PAP1 activity, 25% loss of PAP1 activity at a 1% concentration | Saccharomyces cerevisiae | |
Phenylglyoxal | is an arginine reactive compound, inhibits PAP1 activity in dose-dependent manner | Saccharomyces cerevisiae | |
propranolol | interacts with the Mg2+-binding site of the enzyme, inhibits PAP1 activity in dose-dependent manner | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | colorimetric assay | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.2 | - |
pure enzyme, radioactive assay | Saccharomyces cerevisiae |
5.6 | - |
pure enzyme, colorimetric assay | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dioctanoyl phosphatidic acid + H2O | PAP1 activity is linear with respect to the substrate at concentrations between 0.05-0.8 mM | Saccharomyces cerevisiae | 1,2-dioctanoyl-sn-glycerol + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-sn-phosphatidate phosphohydrolase | - |
Saccharomyces cerevisiae |
Mg2+-dependent PA1 phosphatase | - |
Saccharomyces cerevisiae |
Mg2+-dependent phosphatidate phosphatase | - |
Saccharomyces cerevisiae |
pah1 | - |
Saccharomyces cerevisiae |
PAP1 | - |
Saccharomyces cerevisiae |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.2 | - |
at 30°C, in 50 mM Tris-HCl buffer, pH 7.5, 1 mM MgCl2, and 0.2 mM substrate | Saccharomyces cerevisiae | propranolol | |
1.3 | - |
at 30°C, in 50 mM Tris-HCl buffer, pH 7.5, 1 mM MgCl2, and 0.2 mM substrate | Saccharomyces cerevisiae | Phenylglyoxal |