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Literature summary for 3.1.3.4 extracted from

  • Morris, K.E.; Schang, L.M.; Brindley, D.N.
    Lipid phosphate phosphatase-2 activity regulates S-phase entry of the cell cycle in Rat2 fibroblasts (2006), J. Biol. Chem., 281, 9297-9306.
    View publication on PubMed

Application

Application Comment Organism
additional information fibroblasts that overexpress LPP1 enter S-phase at approximately the same time as vector control cells Mus musculus
additional information fibroblasts that overexpress LPP3 enter S-phase at approximately the same time as vector control cells Rattus norvegicus
additional information increasing LPP2 activity causes premature entry into S-phase, premature cyclin A expression and decreased rates of proliferation at high passage and accumulation in G2/M. Cells transduced with LPP2 activate the G2/M checkpoint and show characteristics of senescence at high passage Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
functional overexpression of GFP-tagged LPPs in rat2 fibroblasts, the transformed cells show reduced proliferation, overview Homo sapiens
rat2 fibroblasts transduced and overexpressed with LPP1 and mLPP1-GFP Mus musculus
rat2 fibroblasts transduced and overexpressed with LPP2, LPP2-GFP and mutant LPP2(R214K)-GFP Homo sapiens
rat2 fibroblasts transduced and overexpressed with LPP3-GFP or myc-LPP3 Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information knock-down of LPPs in rat2 fibroblasts, phenotype, overview Homo sapiens
R214K site-directed mutagenesis Homo sapiens
R214K knock-down of endogenous LPP2 in fibroblasts delays cyclin A accumulation and entry into S-phase of the cell cycle. Cells overexpressing LPP2(R214K)-GFP show no significant change in LPP activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
siRNA rat2 fibroblasts treated with siRNA for LPP2 show a ca. 60% decrease in mRNA for the targeted LPP Homo sapiens
siRNA rat2 fibroblasts treated with siRNA for LPP1 show about a 60% decrease in mRNA for the targeted LPP Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum wild-type and mutant Homo sapiens 5783
-
endosome early stage, wild-type and mutant Homo sapiens 5768
-
membrane
-
Homo sapiens 16020
-
additional information LLP2 is not localized in Golgi apparatus, mitochondria, nucleus, or nuclear membrane Homo sapiens
-
-
additional information not localized to the Golgi apparatus, mitochondria, nucleus, or nuclear membrane Homo sapiens
-
-
plasma membrane
-
Homo sapiens 5886
-
plasma membrane wild-type and mutant Homo sapiens 5886
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
immunoprecipitation, band only visible at mRNA overexpression levels of 100fold or more Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens increasing LPP2 activity causes premature cyclin A expression and decreased LPP2 expression delays cyclin A expression, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-
Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lyso-phosphatidic acid + H2O
-
Mus musculus monoacylglycerol + phosphate
-
?
lyso-phosphatidic acid + H2O
-
Homo sapiens monoacylglycerol + phosphate
-
?
lyso-phosphatidic acid + H2O
-
Rattus norvegicus monoacylglycerol + phosphate
-
?
additional information increasing LPP2 activity causes premature cyclin A expression and decreased LPP2 expression delays cyclin A expression, overview Homo sapiens ?
-
?
phosphatidic acid + H2O
-
Mus musculus diacylglycerol + phosphate
-
?
phosphatidic acid + H2O
-
Homo sapiens diacylglycerol + phosphate
-
?
phosphatidic acid + H2O
-
Rattus norvegicus diacylglycerol + phosphate
-
?
sphingosine 1-phosphate + H2O
-
Mus musculus sphingosine + phosphate
-
?
sphingosine 1-phosphate + H2O
-
Homo sapiens sphingosine + phosphate
-
?
sphingosine 1-phosphate + H2O
-
Rattus norvegicus sphingosine + phosphate
-
?

Synonyms

Synonyms Comment Organism
lipid phosphate phosphatase-2
-
Homo sapiens
LPP1
-
Mus musculus
LPP2
-
Homo sapiens
LPP3
-
Rattus norvegicus