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Literature summary for 3.1.3.4 extracted from
- Enzymological properties of the LPP1-encoded lipid phosphatase from Saccharomyces cerevisiae (2000), Biochim. Biophys. Acta, 1484, 71-82.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Sf-9 insect cells | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | IC50: 15 mM, reaction with phosphatidic acid. IC50: 7.1 mM, reaction with diacylglycerol diphosphate. IC50: 8.2 mM, reaction with lysophosphatidic acid | Saccharomyces cerevisiae |  |
| Co2+ | IC50: 0.029 mM, reaction with phosphatidic acid. IC50: 1.1 mM, reaction with diacylglycerol diphosphate. IC50: 1.2 mM, reaction with lysophosphatidic acid | Saccharomyces cerevisiae | |
| diacylglycerol diphosphate | competitive with respect to phosphatidic acid | Saccharomyces cerevisiae | |
| HgCl2 | - |
Saccharomyces cerevisiae | |
| Mg2+ | inhibition of reaction with diacylglycerol diphosphate. Little effect on reaction with lysophosphatidic acid and phosphatidic acid | Saccharomyces cerevisiae | |
| Mn2+ | IC50: 0.066 mM, reaction with phosphatidic acid. IC50: 0.01 mM, reaction with diacylglycerol diphosphate. IC50: 0.091 mM, reaction with lysophosphatidic acid | Saccharomyces cerevisiae | |
| NaF | 5 mM, 21% inhibition of reaction with phosphatidic acid, 30% inhibition of reaction with diacylglycerol diphosphate and 44% inhibition of the reaction with lysophosphatidic acid | Saccharomyces cerevisiae | |
| NEM | IC50: 0.23 mM, reaction with phosphatidic acid. IC50: 0.12 mM, reaction with diacylglycerol diphosphate. IC50: 0.17 mM, reaction with lysophosphatidic acid | Saccharomyces cerevisiae | |
| p-chloromercuriphenylsulfonic acid | - |
Saccharomyces cerevisiae | |
| Phenylglyoxal | IC50: 7 mM, reaction with phosphatidic acid. IC50: 3.4 mM, reaction with diacylglycerol diphosphate. IC50: 2.5 mM, reaction with lysophosphatidic acid | Saccharomyces cerevisiae | |
| phosphatidic acid | competitive with respect to diacylglycerol diphosphate | Saccharomyces cerevisiae | |
| propranolol | IC50: 2.51 mM, reaction with phosphatidic acid. IC50: 1.68 mM, reaction with diacylglycerol diphosphate. IC50: 7.17 mM, reaction with lysophosphatidic acid | Saccharomyces cerevisiae | |
| ZnCl2 | - |
Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km-value for phosphatidic acid: 0.05 mol%, Km-value for diacylglycerol diphosphate: 0.07 mol%, KM-value for lysophosphatidate : 0.08 mol% | Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | associated with | Saccharomyces cerevisiae | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | Mg2+-independent enzyme | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
two genes DPP1 and LPP1 encode Mg2+-independent phosphatidic acid phosphatase activities | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diacylglycerol diphosphate + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| lysophosphatidate + H2O | - |
Saccharomyces cerevisiae | monoacylglycerol + phosphate | - |
? | |
| phosphatidate + H2O | - |
Saccharomyces cerevisiae | 1,2-diacyl-sn-glycerol + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LPP1-encoded lipid phosphatase | - |
Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
reaction with diacylglycerol diphosphate or lysophosphatidate | Saccharomyces cerevisiae |
| 7.5 | - |
reaction with phosphatidic acid | Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | pH 5.0: about 40% of maximal activity, pH 8.0: about 40% of maximal activity, reaction with lysophosphatidic acid | Saccharomyces cerevisiae |
| 5.5 | 9 | pH 5.0: about 55% of maximal activity, pH 9.0: about 80% of maximal activity, reaction with phosphatidic acid | Saccharomyces cerevisiae |
| 5.5 | 8 | pH 5.0: about 45% of maximal activity, pH 8.0: about 35% of maximal activity, reaction with diacylglycerol diphosphate | Saccharomyces cerevisiae |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Ki-value for phosphatidic acid competitive with respect to diacylglycerol diphosphate : 0.12 mol%. Ki-value for diacylglycerol diphosphate competitive with respect to phosphatidic acid: 0.12 mol% | Saccharomyces cerevisiae |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.091 | - |
IC50: 0.066 mM, reaction with phosphatidic acid. IC50: 0.01 mM, reaction with diacylglycerol diphosphate. IC50: 0.091 mM, reaction with lysophosphatidic acid | Saccharomyces cerevisiae | Mn2+ | |
| 0.17 | - |
IC50: 0.23 mM, reaction with phosphatidic acid. IC50: 0.12 mM, reaction with diacylglycerol diphosphate. IC50: 0.17 mM, reaction with lysophosphatidic acid | Saccharomyces cerevisiae | NEM | |
| 1.2 | - |
IC50: 0.029 mM, reaction with phosphatidic acid. IC50: 1.1 mM, reaction with diacylglycerol diphosphate. IC50: 1.2 mM, reaction with lysophosphatidic acid | Saccharomyces cerevisiae | Co2+ | |
| 2.5 | - |
IC50: 7 mM, reaction with phosphatidic acid. IC50: 3.4 mM, reaction with diacylglycerol diphosphate. IC50: 2.5 mM, reaction with lysophosphatidic acid | Saccharomyces cerevisiae | Phenylglyoxal | |
| 7.17 | - |
IC50: 2.51 mM, reaction with phosphatidic acid. IC50: 1.68 mM, reaction with diacylglycerol diphosphate. IC50: 7.17 mM, reaction with lysophosphatidic acid | Saccharomyces cerevisiae | propranolol | |
| 8.2 | - |
IC50: 15 mM, reaction with phosphatidic acid. IC50: 7.1 mM, reaction with diacylglycerol diphosphate. IC50: 8.2 mM, reaction with lysophosphatidic acid | Saccharomyces cerevisiae | Ca2+ | |
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